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UGT2B10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UGT2B10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119921-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UGT2B10 (NM_001075), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
UDPGT 2B10 | UGT2B10 | UDPGT2B10
Origin Species
Human
NCBI GenBank
NM_001075
SwissProt
P36537

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALKWTTVLL IQLSFYFSSG SCGKVLVWAA EYSLWMNMKT ILKELVQRGH EVTVLASSAS
61ILFDPNDSST LKLEVYPTSL TKTEFENIIM QLVKRLSEIQ KDTFWLPFSQ EQEILWAIND
121IIRNFCKDVV SNKKLMKKLQ ESRFDIVFAD AYLPCGELLA ELFNIPFVYS HSFSPGYSFE
181RHSGGFIFPP SYVPVVMSKL SDQMTFMERV KNMLYVLYFD FWFQIFNMKK WDQFYSEVLG
241RPTTLSETMR KADIWLMRNS WNFKFPHPFL PNVDFVGGLH CKPAKPLPKE MEEFVQSSGE
301NGVVVFSLGS MVSNMTEERA NVIATALAKI PQKVLWRFDG NKPDALGLNT RLYKWIPQND
361LLGHPKTRAF ITHGGANGIY EAIYHGIPMV GIPLFFDQPD NIAHMKAKGA AVRVDFNTMS
421STDLLNALKT VINDPSYKEN IMKLSRIQHD QPVKPLDRAV FWIEFVMRHK GAKHLRVAAH
481NLTWFQYHSL DVIGFLLACV ATVLFIITKC CLFCFWKFAR KGKKGKRD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

528 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.