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UGT2A2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UGT2A2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124173-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UGT2A2 (NM_001105677), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001105677

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPKKFVQMLV FNLTLTEVVL SGNVLIWPTD GSHWLNIKII LEELIQRNHN VTVLASSATL
61FINSNPDSPV NFEVIPVSYK KSNIDSLIEH MIMLWIDHRP TPLTIWAFYK ELGKLLDTFF
121QINIQLCDGV LKNPKLMARL QKGGFDVLVA DPVTICGDLV ALKLGIPFMY TLRFSPASTV
181ERHCGKIPAP VSYVPAALSE LTDQMTFGER IKNTISYSLQ DYIFQSYWGE WNSYYSKILG
241RPTTLCETMG KAEIWLIRTY WDFEFPRPYL PNFEFVGGLH CKPAKPLPKE MEEFIQSSGK
301NGVVVFSLGS MVKNLTEEKA NLIASALAQI PQKVLWRYKG KKPATLGNNT QLFDWIPQND
361LLGHPKTKAF ITHGGTNGIY EAIYHGVPMV GVPMFADQPD NIAHMKAKGA AVEVNLNTMT
421SVDLLSALRT VINEPSYKEN AMRLSRIHHD QPVKPLDRAV FWIEFVMRHK GAKHLRVAAH
481DLTWFQYHSL DVIGFLLVCV TTAIFLVIQC CLFSCQKFGK IGKKKKRE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

528 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.