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UGT1A3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UGT1A3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111434-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UGT1A3 (NM_019093), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GNT1 | UDPGT | UGT1A3 | UDPGT 1-3 | UGT1C | UGT1 | UGT1*3 | UGT-1C | UGT1-03 | UGT1.3
Origin Species
Human
NCBI GenBank
NM_019093
SwissProt
P35503

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATGLQVPLP WLATGLLLLL SVQPWAESGK VLVVPIDGSH WLSMREVLRE LHARGHQAVV
61LTPEVNMHIK EENFFTLTTY AISWTQDEFD RHVLGHTQLY FETEHFLKKF FRSMAMLNNM
121SLVYHRSCVE LLHNEALIRH LNATSFDVVL TDPVNLCAAV LAKYLSIPTV FFLRNIPCDL
181DFKGTQCPNP SSYIPRLLTT NSDHMTFMQR VKNMLYPLAL SYICHAFSAP YASLASELFQ
241REVSVVDILS HASVWLFRGD FVMDYPRPIM PNMVFIGGIN CANRKPLSQE FEAYINASGE
301HGIVVFSLGS MVSEIPEKKA MAIADALGKI PQTVLWRYTG TRPSNLANNT ILVKWLPQND
361LLGHPMTRAF ITHAGSHGVY ESICNGVPMV MMPLFGDQMD NAKRMETKGA GVTLNVLEMT
421SEDLENALKA VINDKSYKEN IMRLSSLHKD RPVEPLDLAV FWVEFVMRHK GAPHLRPAAH
481DLTWYQYHSL DVIGFLLAVV LTVAFITFKC CAYGYRKCLG KKGRVKKAHK SKTH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

534 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.