Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UGGT1 / UGGT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111343-100
Catalog Number: LS-G111343
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UGGT(UGGT1) (NM_020120), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GT | HUGT1 | UGGT | UGTR | UGGT1 | UGT1 | UGCGL1
- Origin Species
- Human
- NCBI GenBank
- NM_020120
- SwissProt
- Q9NYU2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 177 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGCKGDASGA CAAGALPVTG VCYKMGVLVV LTVLWLFSSV KADSKAITTS LTTKWFSTPL61LLEASEFLAE DSQEKFWNFV EASQNIGSSD HDGTDYSYYH AILEAAFQFL SPLQQNLFKF121CLSLRSYSAT IQAFQQIAAD EPPPEGCNSF FSVHGKKTCE SDTLEALLLT ASERPKPLLF181KGDHRYPSSN PESPVVIFYS EIGSEEFSNF HRQLISKSNA GKINYVFRHY IFNPRKEPVY241LSGYGVELAI KSTEYKAKDD TQVKGTEVNT TVIGENDPID EVQGFLFGKL RDLHPDLEGQ301LKELRKHLVE STNEMAPLKV WQLQDLSFQT AARILASPVE LALVVMKDLS QNFPTKARAI361TKTAVSSELR TEVEENQKYF KGTLGLQPGD SALFINGLHM DLDTQDIFSL FDVLRNEARV421MEGLHRLGIE GLSLHNVLKL NIQPSEADYA VDIRSPAISW VNNLEVDSRY NSWPSSLQEL481LRPTFPGVIR QIRKNLHNMV FIVDPAHETT AELMNTAEMF LSNHIPLRIG FIFVVNDSED541VDGMQDAGVA VLRAYNYVAQ EVDDYHAFQT LTHIYNKVRT GEKVKVEHVV SVLEKKYPYV601EVNSILGIDS AYDRNRKEAR GYYEQTGVGP LPVVLFNGMP FEREQLDPDE LETITMHKIL661ETTTFFQRAV YLGELPHDQD VVEYIMNQPN VVPRINSRIL TAERDYLDLT ASNNFFVDDY721ARFTILDSQG KTAAVANSMN YLTKKGMSSK EIYDDSFIRP VTFWIVGDFD SPSGRQLLYD781AIKHQKSSNN VRISMINNPA KEISYENTQI SRAIWAALQT QTSNAAKNFI TKMAKEGAAE841ALAAGADIAE FSVGGMDFSL FKEVFESSKM DFILSHAVYC RDVLKLKKGQ RAVISNGRII901GPLEDSELFN QDDFHLLENI ILKTSGQKIK SHIQQLRVEE DVASDLVMKV DALLSAQPKG961DPRIEYQFFE DRHSAIKLRP KEGETYFDVV AVVDPVTREA QRLAPLLLVL AQLINMNLRV1021FMNCQSKLSD MPLKSFYRYV LEPEISFTSD NSFAKGPIAK FLDMPQSPLF TLNLNTPESW1081MVESVRTPYD LDNIYLEEVD SVVAAEYELE YLLLEGHCYD ITTGQPPRGL QFTLGTSANP1141VIVDTIVMAN LGYFQLKANP GAWILRLRKG RSEDIYRIYS HDGTDSPPDA DEVVIVLNNF1201KSKIIKVKVQ KKADMVNEDL LSDGTSENES GFWDSFKWGF TGQKTEEVKQ DKDDIINIFS1261VASGHLYERF LRIMMLSVLK NTKTPVKFWF LKNYLSPTFK EFIPYMANEY NFQYELVQYK1321WPRWLHQQTE KQRIIWGYKI LFLDVLFPLV VDKFLFVDAD QIVRTDLKEL RDFNLDGAPY1381GYTPFCDSRR EMDGYRFWKS GYWASHLAGR KYHISALYVV DLKKFRKIAA GDRLRGQYQG1441LSQDPNSLSN LDQDLPNNMI HQVPIKSLPQ EWLWCETWCD DASKKRAKTI DLCNNPMTKE1501PKLEAAVRIV PEWQDYDQEI KQLQIRFQKE KETGALYKEK TKEPSREGPQ KREELTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1555 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.