Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UGDH / UDPGDH Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129797-100
Catalog Number: LS-G129797
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UDP glucose dehydrogenase(UGDH) (NM_001184700), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- UDP-GlcDH | UDPGDH | UDP-Glc dehydrogenase | UGD | UDP-glucose 6-dehydrogenase | UGDH | GDH | UDP-glucose dehydrogenase
- Origin Species
- Human
- NCBI GenBank
- NM_003359
- SwissProt
- O60701
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDVNES RINAWNSPTL PIYEPGLKEV61VESCRGKNLF FSTNIDDAIK EADLVFISVL SNPEFLAEGT AIKDLKNPDR VLIGGDETPE121GQRAVQALCA VYEHWVPREK ILTTNTWSSE LSKLAANAFL AQRISSINSI SALCEATGAD181VEEVATAIGM DQRIGNKFLK ASVGFGGSCF QKDVLNLVYL CEALNLPEVA RYWQQVIDMN241DYQRRRFASR IIDSLFNTVT DKKIAILGFA FKKDTGDTRE SSSIYISKYL MDEGAHLHIY301DPKVPREQIV VDLSHPGVSE DDQVSRLVTI SKDPYEACDG AHAVVICTEW DMFKELDYER361IHKKMLKPAF IFDGRRVLDG LHNELQTIGF QIETIGKKVS SKRIPYAPSG EIPKFSLQDP421PNKKPKVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
427 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.