Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UBXN6 / UBXD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109496-100
Catalog Number: LS-G109496
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBXN6 (NM_025241), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- UBX domain-containing 1 | UBX domain-containing 2 | UBXD1 | UBX domain containing 1 | UBX domain protein 6 | UBXDC2 | UBXN6
- Origin Species
- Human
- NCBI GenBank
- NM_025241
- SwissProt
- Q9BZV1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKKFFQEFKA DIKFKSAGPG QKLKESVGEK AHKEKPNQPA PRPPRQGPTN EAQMAAAAAL61ARLEQKQSRA WGPTSQDTIR NQVRKELQAE ATVSGSPEAP GTNVVSEPRE EGSAHLAVPG121VYFTCPLTGA TLRKDQRDAC IKEAILLHFS TDPVAASIMK IYTFNKDQDR VKLGVDTIAK181YLDNIHLHPE EEKYRKIKLQ NKVFQERINC LEGTHEFFEA IGFQKVLLPA QDQEDPEEFY241VLSETTLAQP QSLERHKEQL LAAEPVRAKL DRQRRVFQPS PLASQFELPG DFFNLTAEEI301KREQRLRSEA VERLSVLRTK AMREKEEQRG LRKYNYTLLR VRLPDGCLLQ GTFYARERLG361AVYGFVREAL QSDWLPFELL ASGGQKLSED ENLALNECGL VPSALLTFSW DMAVLEDIKA421AGAEPDSILK PELLSAIEKL LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
441 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.