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UBXN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBXN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101346-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBXN1 (NM_015853), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
2B28 | SAPK substrate protein 1 | UBXD10 | UBX domain protein 1 | LOC51035 | SAKS1 | UBA/UBX 33.3 kDa protein | UBXN1
Origin Species
Human
NCBI GenBank
NM_015853
SwissProt
Q04323

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
34.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELTALESL IEMGFPRGRA EKALALTGNQ GIEAAMDWLM EHEDDPDVDE PLETPLGHIL
61GREPTSSEQG GLEGSGSAAG EGKPALSEEE RQEQTKRMLE LVAQKQRERE EREEREALER
121ERQRRRQGQE LSAARQRLQE DEMRRAAEER RREKAEELAA RQRVREKIER DKAERAKKYG
181GSVGSQPPPV APEPGPVPSS PSQEPPTKRE YDQCRIQVRL PDGTSLTQTF RAREQLAAVR
241LYVELHRGEE LGGGQDPVQL LSGFPRRAFS EADMERPLQE LGMAARLETR TRNWGSREAC
301LGKGGMQREG ALTRTRPLEQ KLISEEDLAA NDILDYKDDD DKV

343 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.