Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UBXD2 / UBXN4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113827-100
Catalog Number: LS-G113827
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBXD2(UBXN4) (NM_014607), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Erasin | KIAA0242 | UBX domain-containing 1 | UBX domain-containing 2 | UBXD2 | UBXN4 | UBXDC1 | UBX domain containing 2 | UBX domain protein 4
- Origin Species
- Human
- NCBI GenBank
- D87684
- SwissProt
- Q92575
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLWFQGAIPA AIATAKRSGA VFVVFVAGDD EQSTQMAASW EDDKVTEASS NSFVAIKIDT61KSEACLQFSQ IYPVVCVPSS FFIGDSGIPL EVIAGSVSAD ELVTRIHKVR QMHLLKSETS121VANGSQSESS VSTPSASFEP NNTCENSQSR NAELCEIPPT SDTKSDTATG GESAGHATSS181QEPSGCSDQR PAEDLNIRVE RLTKKLEERR EEKRKEEEQR EIKKEIERRK TGKEMLDYKR241KQEEELTKRM LEERNREKAE DRAARERIKQ QIALDRAERA ARFAKTKEEV EAAKAAALLA301KQAEMEVKRE SYARERSTVA RIQFRLPDGS SFTNQFPSDA PLEEARQFAA QTVGNTYGNF361SLATMFPRRE FTKEDYKKKL LDLELAPSAS VVLLPAGRPT ASIVHSSSGD IWTLLGTVLY421PFLAIWRLIS NFLFSNPPPT QTSVRVTSSE PPNPASSSKS EKREPVRKRV LEKRGDDFKK481EGKIYRLRTQ DDGEDENNTW NGNSTQQMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
508 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.