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UBQLN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBQLN3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112439-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBQLN3 (NM_017481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TUP-1 | Ubiquilin 3 | UBQLN3 | Ubiquilin-3
Origin Species
Human
NCBI GenBank
NM_017481
SwissProt
Q9H347

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKGGEALPQ GSPAPVQDPH LIKVTVKTPK DKEDFSVTDT CTIQQLKEEI SQRFKAHPDQ
61LVLIFAGKIL KDPDSLAQCG VRDGLTVHLV IKRQHRAMGN ECPAASVPTQ GPSPGSLPQP
121SSIYPADGPP AFSLGLLTGL SRLGLAYRGF PDQPSSLMRQ HVSVPEFVTQ LIDDPFIPGL
181LSNTGLVRQL VLDNPHMQQL IQHNPEIGHI LNNPEIMRQT LEFLRNPAMM QEMIRSQDRV
241LSNLESIPGG YNVLCTMYTD IMDPMLNAVQ EQFGGNPFAT ATTDNATTTT SQPSRMENCD
301PLPNPWTSTH GGSGSRQGRQ DGDQDAPDIR NRFPNFLGII RLYDYLQQLH ENPQSLGTYL
361QGTASALSQS QEPPPSVNRV PPSSPSSQEP GSGQPLPEES VAIKGRSSCP AFLRYPTENS
421TGQGGDQDGA GKSSTGHSTN LPDLVSGLGD SANRVPFAPL SFSPTAAIPG IPEPPWLPSP
481AYPRSLRPDG MNPAPQLQDE IQPQLPLLMH LQAAMANPRA LQALRQIEQG LQVLATEAPR
541LLLWFMPCLA GTGSVAGGIE SREDPLMSED PLPNPPPEVF PALDSAELGF LSPPFLHMLQ
601DLVSTNPQQL QPEAHFQVQL EQLRSMGFLN REANLQALIA TGGDVDAAVE KLRQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

655 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.