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UBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UBP1 antibodies →
$504.00each
Catalog Number: LS-G113884-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Upstream Binding Protein 1(UBP1) (NM_014517), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LBP1 | LBP-1a | LBP1A | LBP1B | Transcription factor LBP-1 | Upstream-binding protein 1 | LBP-1B | UBP1
Origin Species
Human
NCBI GenBank
NM_014517
SwissProt
Q9NZI7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MAWVLKMDEV IESGLVHDFD ASLSGIGQEL GAGAYSMSDV LALPIFKQED SSLPLDGETE
61HPPFQYVMCA ATSPAVKLHD ETLTYLNQGQ SYEIRMLDNR KMGDMPEING KLVKSIIRVV
121FHDRRLQYTE HQQLEGWKWN RPGDRLLDLD IPMSVGIIDT RTNPSQLNAV EFLWDPAKRT
181SAFIQVHCIS TEFTPRKHGG EKGVPFRIQV DAFKQNENGE YTDHLHSASC QIKVFKPKGA
241DRKQKTDREK MEKRTAHEKE KYQPSYDTTI LTEMRLEPII EDAVEHEQKK SSKRTLPADY
301GDSLAKRGSC SPWPDAPTAY VNNSPSPAPT FTSPQQSTCS VPDSNSSSPN HQGDGASQTS
361GEQIQPSATI QETQQWLLKN RFSSYTRLFS NFSGADLLKL TKEDLVQICG AADGIRLYNS
421LKSRSVRPRL TIYVCREQPS STVLQGQQQA ASSASENGSG APYVYHAIYL EEMIASEVAR
481KLALVFNIPL HQINQVYRQG PTGIHILVSD QMVQNFQDES CFLFSTVKAE SSDGIHIILK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

540 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.