Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UBN1 / Ubinuclein 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120077-100
Catalog Number: LS-G120077
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBN1 (NM_001079514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HIRA-binding protein | Protein VT4 | UBN1 | VT | Ubinuclein-1 | VT4 | Ubinuclein 1
- Origin Species
- Human
- NCBI GenBank
- NM_016936
- SwissProt
- Q9NPG3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 121.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEPHRVQFT SLPGSLNPAF LKKSRKEEAG AGEQHQDCEP AAAAVRITLT LFEPDHKRCP61EFFYPELVKN IRGKVKGLQP GDKKKDLSDP FNDEEKERHK VEALARKFEE KYGGKKRRKD121RIQDLIDMGY GYDESDSFID NSEAYDELVP ASLTTKYGGF YINSGTLQFR QASESEDDFI181KEKKKKSPKK RKLKEGGEKI KKKKKDDTYD KEKKSKKSKF SKAGFTALNA SKEKKKKKYS241GALSVKEMLK KFQKEKEAQK KREEEHKPVA VPSAEAQGLR ELEGASDPLL SLFGSTSDND301LLQAATAMDS LTDLDLEHLL SESPEGSPFR DMDDGSDSLG VGLDQEFRQP SSLPEGLPAP361LEKRVKELAQ AARAAEGESR QKFFTQDING ILLDIEAQTR ELSSQVRSGV YAYLASFLPC421SKDALLKRAR KLHLYEQGGR LKEPLQKLKE AIGRAMPEQM AKYQDECQAH TQAKVAKMLE481EEKDKEQRDR ICSDEEEDEE KGGRRIMGPR KKFQWNDEIR ELLCQVVKIK LESQDLERNN541KAQAWEDCVK GFLDAEVKPL WPKGWMQART LFKESRRGHG HLTSILAKKK VMAPSKIKVK601ESSTKPDKKV SVPSGQIGGP IALPSDHQTG GLSIGASSRE LPSQASGGLA NPPPVNLEDS661LDEDLIRNPA SSVEAVSKEL AALNSRAAGN SEFTLPAPSK APAEKVGGVL CTEEKRNFAK721PSPSAPPPAS SLQSPLNFLA EQALALGQSS QEKKPESSGY KELSCQAPLN KGLPEVHQSK781AKHHSLPRTS HGPQVAVPVP GPQVKVFHAG TQQQKNFTPP SPFANKLQGP KASPTQCHRS841LLQLVKTAAK GQGFHPSAPA TSGGLSASSS SSHKTPASSS SALSHPAKPH SVSSAGSSYK901NNPFASSISK HGVSSGSSSS GGTPVQSSVS GSLVPGIQPP SVGQATSRPV PSSAGKKMPV961SQKLTLVAPP GGPNGDSSGG TQGVAKLLTS PSLKPSAVSS VTSSTSLSKG ASGTVLLAGS1021SLMASPYKSS SPKLSGAMSS NSLGIITPVP IPVHVLSFSA DSSAKAGVSK DAIVTGPAPG1081SFHHGLGHSL LAGLHSSPPH AAPLPHAAVP THIPQSLPGA SQLHGKGPAV PRKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1134 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.