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UBE3B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBE3B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UBE3B antibodies →
$725.00each
Catalog Number: LS-G107623-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBE3B (NM_130466), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
UBE3B | Ubiquitin-protein ligase E3B | Ubiquitin protein ligase E3B
Origin Species
Human
SwissProt
Q7Z3V4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
122.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MFTLSQTSRA WFIDRARQAR EERLVQKERE RAAVVIQAHV RSFLCRSRLQ RDIRREIDDF
61FKADDPESTK RSALCIFKIA RKLLFLFRIK EDNERFEKLC RSILSSMDAE NEPKVWYVSL
121ACSKDLTLLW IQQIKNILWY CCDFLKQLKP EILQDSRLIT LYLTMLVTFT DTSTWKILRG
181KGESLRPAMN HICANIMGHL NQHGFYSVLQ ILLTRGLARP RPCLSKGTLT AAFSLALRPV
241IAAQFSDNLI RPFLIHIMSV PALVTHLSTV TPERLTVLES HDMLRKFIIF LRDQDRCRDV
301CESLEGCHTL CLMGNLLHLG SLSPRVLEEE TDGFVSLLTQ TLCYCQKYVS QKKSNLTHWH
361PVLGWFSQSV DYGLNESMHL ITKQLQFLWG VPLIRIFFCD ILSKKLLESQ EPAHAQPASP
421QNVLPVKSLL KRAFQKSASV RNILRPVGGK RVDSAEVQKV CNICVLYQTS LTTLTQIRLQ
481ILTGLTYLDD LLPKLWAFIC ELGPHGGLKL FLECLNNDTE ESKQLLAMLM LFCDCSRHLI
541TILDDIEVYE EQISFKLEEL VTISSFLNSF VFKMIWDGIV ENAKGETLEL FQSVHGWLMV
601LYERDCRRRF TPEDHWLRKD LKPSVLFQEL DRDRKRAQLI LQYIPHVIPH KNRVLLFRTM
661VTKEKEKLGL VETSSASPHV THITIRRSRM LEDGYEQLRQ LSQHAMKGVI RVKFVNDLGV
721DEAGIDQDGV FKEFLEEIIK RVFDPALNLF KTTSGDERLY PSPTSYIHEN YLQLFEFVGK
781MLGKAVYEGI VVDVPFASFF LSQLLGHHHS VFYSSVDELP SLDSEFYKNL TSIKRYDGDI
841TDLGLTLSYD EDVMGQLVCH ELIPGGKTIP VTNENKISYI HLMAHFRMHT QIKNQTAALI
901SGFRSIIKPE WIRMFSTPEL QRLISGDNAE IDLEDLKKHT VYYGGFHGSH RVIIWLWDIL
961ASDFTPDERA MFLKFVTSCS RPPLLGFAYL KPPFSIRCVE VSDDQDTGDT LGSVLRGFFT
1021IRKREPGGRL PTSSTCFNLL KLPNYSKKSV LREKLRYAIS MNTGFELS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1068 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.