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UBAP2L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBAP2L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UBAP2L antibodies →
$504.00each
Catalog Number: LS-G113639-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBAP2L (NM_014847), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NICE-4 | KIAA0144 | UBAP2L | NICE4
Origin Species
Human
NCBI GenBank
NM_014847
SwissProt
Q14157

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
114.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMTSVGTNRA RGNWEQPQNQ NQTQHKQRPQ ATAEQIRLAQ MISDHNDADF EEKVKQLIDI
61TGKNQDECVI ALHDCNGDVN RAINVLLEGN PDTHSWEMVG KKKGVSGQKD GGQTESNEEG
121KENRDRDRDY SRRRGGPPRR GRGASRGREF RGQENGLDGT KSGGPSGRGT ERGRRGRGRG
181RGGSGRRGGR FSAQGMGTFN PADYAEPANT DDNYGNSSGN TWNNTGHFEP DDGTSAWRTA
241TEEWGTEDWN EDLSETKIFT ASNVSSVPLP AENVTITAGQ RIDLAVLLGK TPSTMENDSS
301NLDPSQAPSL AQPLVFSNSK QTAISQPASG NTFSHHSMVS MLGKGFGDVG EAKGGSTTGS
361QFLEQFKTAQ ALAQLAAQHS QSGSTTTSSW DMGSTTQSPS LVQYDLKNPS DSAVHSPFTK
421RQAFTPSSTM MEVFLQEKSP AVATSTAAPP PPSSPLPSKS TSAPQMSPGS SDNQSSSPQP
481AHQKLKQQKK KASLTSKIPA LAVEMPGSAD ISGLNLQFGA LQFGSEPVLS DYESTPTTSA
541SSSQAPSSLY TSTASESSST ISSNQSQESG YQSGPIQSTT YTSQNNAQGP LYEQRSTQTR
601RYPSSISSSP QKDLTQAKNG FSSVQATQLQ TTQSVEGATG SAVKSDSPST SSIPPLNETV
661SAASLLTTTN QHSSSLGGLS HSEEIPNTTT TQHSSTLSTQ QNTLSSSTSS GRTSTSTLLH
721TSVESEANLH SSSSTFSTTS STVSAPPPVV SVSSSLNSGS SLGLSLGSNS TVTASTRSSV
781ATTSGKAPPN LPPGVPPLLP NPYIMAPGLL HAYPPQVYGY DDLQMLQTRF PLDYYSIPFP
841TPTTPLTGRD GSLASNPYSG DLTKFGRGDA SSPAPATTLA QPQQNQTQTH HTTQQTFLNP
901ALPPGYSYTS LPYYTGVPGL PSTFQYGPAV FPVAPTSSKQ HGVNVSVNAS ATPFQQPSGY
961GSHGYNTGVS VTSSNTGVPD ISGSVYSKTQ QSFEKQGFHS GTPAASFNLP SALGSGGPIN
1021PATAAAYPPA PFMHILTPHQ QPHSQILHHH LQQDGQTGSG QRSQTSSIPQ KPQTNKSAYN
1081SYSWGANTRT RPLEQKLISE EDLAANDILD YKDDDDKV

1118 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.