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UBAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UBAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111709-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBAP2 (NM_018449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA176F3.5 | AD-012 protein | UBAP2 | Ubiquitin associated protein 2 | UBAP-2 | Ubiquitin-associated protein 2 | KIAA1491
Origin Species
Human
NCBI GenBank
NM_018449
SwissProt
Q5T6F2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMTSVSSDHC RGAREKPQIS AAQSTQPQKQ VVQATAEQMR LAQVIFDKND SDFEAKVKQL
61MEVTGKNQDE CIVALHDCNG DVNKAINILL EGNSDTTSWE TVGCKKKNFA KENSENKENR
121EKKSEKESSR GRGNNNRKGR GGNRGREFRG EENGIDCNQV DKPSDRGKRA RGRGFGRGRG
181RGAGRFSTQG MGTFNPADYS DSTSTDVCGT KLVVWEAAQN GADEGTELAS NTHNIAQDLS
241NKSSYGLKGA WKNSVEEWTT EDWTEDLSET KVFTASSAPA ENHILPGQSI DLVALLQKPV
301PHSQASEANS FETSQQQGFG QALVFTNSQH NNQMAPGTGS STAVNSCSPQ SLSSVLGSGF
361GELAPPKMAN ITSSQILDQL KAPSLGQFTT TPSTQQNSTS HPTTTTSWDL KPPTSQSSVL
421SHLDFKSQPE PSPVLSQLSQ RQQHQSQAVT VPPPGLESFP SQAKLRESTP GDSPSTVNKL
481LQLPSTTIEN ISVSVHQPQP KHIKLAKRRI PPASKIPASA VEMPGSADVT GLNVQFGALE
541FGSEPSLSEF GSAPSSENSN QIPISLYSKS LSEPLNTSLS MTSAVQNSTY TTSVITSCSL
601TSSSLNSASP VAMSSSYDQS SVHNRIPYQS PVSSSESAPG TIMNGHGGGR SQQTLDTPKT
661TGPPSALPSV SSLPSTTSCT ALLPSTSQHT GDLTSSPLSQ LSSSLSSHQS SLSAHAALSS
721STSHTHASVE SASSHQSSAT FSTAATSVSS SASSGASLSS SMNTANSLCL GGTPASASSS
781SSRAAPLVTS GKAPPNLPQG VPPLLHNQYL VGPGGLLPAY PIYGYDELQM LQSRLPVDYY
841GIPFAAPTAL ASRDGSLANN PYPGDVTKFG RGDSASPAPA TTPAQPQQSQ SQTHHTAQQP
901FVNPALPPGY SYTGLPYYTG MPSAFQYGPT MFVPPASAKQ HGVNLSTPTP PFQQASGYGQ
961HGYSTGYDDL TQGTAAGDYS KGGYAGSSQA PNKSAGSGPG KGVSVSSSTT GLPDMTGSVY
1021NKTQTFDKQG FHAGTPPPFS LPSVLGSTGP LASGAAPGYA PPPFLHILPA HQQPHSQLLH
1081HHLPQDAQSG SGQRSQPSSL QPKSQASKPA YGNSPYWTN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1119 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.