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UAP1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UAP1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129018-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UAP1L1 (NM_207309), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RP11-229P13.18 | UAP1L1
Origin Species
Human
SwissProt
Q3KQV9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASEQDVRAR LQRAGQEHLL RFWAELAPEP RAALLAELAL LEPEALREHC RRAAEACARP
61HGPPPDLAAR LRPLPPERVG RASRSDPETR RRWEEEGFRQ ISLNKVAVLL LAGGQGTRLG
121VTYPKGMYRV GLPSRKTLYQ LQAERIRRVE QLAGERHGTR CTVPWYVMTS EFTLGPTAEF
181FREHNFFHLD PANVVMFEQR LLPAVTFDGK VILERKDKVA MAPDGNGGLY CALEDHKILE
241DMERRGVEFV HVYCVDNILV RLADPVFIGF CVLQGADCGA KVVEKAYPEE PVGVVCQVDG
301VPQVVEYSEI SPETAQLRAS DGSLLYNAGN ICNHFFTRGF LKAVTREFEP LLKPHVAVKK
361VPYVDEEGNL VKPLKPNGIK MEKFVFDVFR FAKNFAALEV LREEEFSPLK NAEPADRDSP
421RTARQALLTQ HYRWALRAGA RFLDAHGAWL PELPSLPPNG DPPAICEISP LVSYSGEGLE
481VYLQGREFQS PLILDEDQAR EPQLQES
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

507 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.