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TYSND1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TYSND1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105203-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TYSND1 (NM_173555), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
NET41 | Trypsin domain containing 1 | TYSND1
Origin Species
Human
NCBI GenBank
NM_173555
SwissProt
Q2T9J0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MRRQWGSAMR AAEQAGCMVS ASRAGQPEAG PWSCSGVILS RSPGLVLCHG GIFVPFLRAG
61SEVLTAAGAV FLPGDSCRDD LRLHVQWAPT AAGPGGGAER GRPGLCTPQC ASLEPGPPAP
121SRGRPLQPRL PAELLLLLSC PAFWAHFARL FGDEAAEQWR FSSAARDDEV SEDEEADQLR
181ALGWFALLGV RLGQEEVEEE RGPAMAVSPL GAVPKGAPLL VCGSPFGAFC PDIFLNTLSC
241GVLSNVAGPL LLTDARCLPG TEGGGVFTAR PAGALVALVV APLCWKAGEW VGFTLLCAAA
301PLFRAARDAL HRLPHSTAAL AALLPPEVGV PWGLPLRDSG PLWAAAAVLV ECGTVWGSGV
361AVAPRLVVTC RHVSPREAAR VLVRSTTPKS VAIWGRVVFA TQETCPYDIA VVSLEEDLDD
421VPIPVPAEHF HEGEAVSVVG FGVFGQSCGP SVTSGILSAV VQVNGTPVML QTTCAVHSGS
481SGGPLFSNHS GNLLGIITSN TRDNNTGATY PHLNFSIPIT VLQPALQQYS QTQDLGGLRE
541LDRAAEPVRV VWRLQRPLAE APRSKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

566 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.