Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TXLNA / Alpha Taxilin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104972-100
Catalog Number: LS-G104972
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Alpha Taxilin(TXLNA) (NM_175852), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- IL14 | Interleukin 14 | Alpha-taxilin | TXLNA | Taxilin alpha | RP4-622L5.4 | TXLN
- Origin Species
- Human
- NCBI GenBank
- L15344
- SwissProt
- P40222
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKNQDKKNGA AKQSNPKSSP GQPEAGPEGA QERPSQAAPA VEAEGPGSSQ APRKPEGAQA61RTAQSGALRD VSEELSRQLE DILSTYCVDN NQGGPGEDGA QGEPAEPEDA EKSRTYVARN121GEPEPTPVVN GEKEPSKGDP NTEEIRQSDE VGDRDHRRPQ EKKKAKGLGK EITLLMQTLN181TLSTPEEKLA ALCKKYAELL EEHRNSQKQM KLLQKKQSQL VQEKDHLCGE HSKAVLARSK241LESLCRELQR HNRSLKEEGV QRAREEEEKR KEVTSHFQVT LNDIQLQMEQ HNERNSKLRQ301ENMELAERLK KLIEQYELRE EHIDKVFKHK DLQQQLVDAK LQQAQEMLKE AEERHQREKD361FLLKEAVESQ RMCELMKQQE THLKQQLALY TEKFEEFQNT LSKSSEVFTT FKQEMEKMTK421KIKKLEKETT MYRSRWESSN KALLEMAEEK TVRDKELEGL QVKIQRLEKL CRALQTERND481LNKRVQDLSA GGQGSLTDSG PERRPEGPGA QAPSSPRVTE APCYPGAPST EASGQTGPQE541PTSARATRTRPLEQKLISEEDLAANDILDYKDDDDKV
546 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.