Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TWF1 / PTK9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99888-100
Catalog Number: LS-G99888
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PTK9(TWF1) (NM_002822), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A6 | A6 protein tyrosine kinase | Protein A6 | TWF1 | Twinfilin | Twinfilin-1 | Protein tyrosine kinase | Protein tyrosine kinase 9 | PTK9 | PTK9 protein tyrosine kinase 9
- NCBI GenBank
- NM_002822
- SwissProt
- Q12792
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTRRGRRRSS HFLGPPAGAA GCTQRRSREL AAAAMSHQTG IQASEDVKEI FARARNGKYR61LLKISIENEQ LVIGSYSQPS DSWDKDYDSF VLPLLEDKQP CYILFRLDSQ NAQGYEWIFI121AWSPDHSHVR QKMLYAATRA TLKKEFGGGH IKDEVFGTVK EDVSLHGYKK YLLSQSSPAP181LTAAEEELRQ IKINEVQTDV GVDTKHQTLQ GVAFPISREA FQALEKLNNR QLNYVQLEID241IKNEIIILAN TTNTELKDLP KRIPKDSARY HFFLYKHSHE GDYLESIVFI YSMPGYTCSI301RERMLYSSCK SRLLEIVERQ LQMDVIRKIE IDNGDELTAD FLYEEVHPKQ HAHKQSFAKP361KGPAGKRGIR RLIRGPAETE ATTDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
384 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.