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TULP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TULP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111261-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TULP4 (NM_020245), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RP3-442A17.1 | Tubby-like protein 4 | Tubby-related protein 4 | TULP4 | Tubby like protein 4 | Tubby superfamily protein | TUBL4 | KIAA1397 | Tubby super-family protein | TUSP
Origin Species
Human
NCBI GenBank
NM_020245
SwissProt
Q9NRJ4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
168.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYAAVEHGPV LCSDSNILCL SWKGRVPKSE KEKPVCRRRY YEEGWLATGN GRGVVGVTFT
61SSHCRRDRST PQRINFNLRG HNSEVVLVRW NEPYQKLATC DADGGIFVWI QYEGRWSVEL
121VNDRGAQVSD FTWSHDGTQA LISYRDGFVL VGSVSGQRHW SSEINLESQI TCGIWTPDDQ
181QVLFGTADGQ VIVMDCHGRM LAHVLLHESD GVLGMSWNYP IFLVEDSSES DTDSDDYAPP
241QDGPAAYPIP VQNIKPLLTV SFTSGDISLM NNYDDLSPTV IRSGLKEVVA QWCTQGDLLA
301VAGMERQTQL GELPNGPLLK SAMVKFYNVR GEHIFTLDTL VQRPIISICW GHRDSRLLMA
361SGPALYVVRV EHRVSSLQLL CQQAIASTLR EDKDVSKLTL PPRLCSYLST AFIPTIKPPI
421PDPNNMRDFV SYPSAGNERL HCTMKRTEDD PEVGGPCYTL YLEYLGGLVP ILKGRRISKL
481RPEFVIMDPR TDSKPDEIYG NSLISTVIDS CNCSDSSDIE LSDDWAAKKS PKISRASKSP
541KLPRISIEAR KSPKLPRAAQ ELSRSPRLPL RKPSVGSPSL TRREFPFEDI TQHNYLAQVT
601SNIWGTKFKI VGLAAFLPTN LGAVIYKTSL LHLQPRQMTI YLPEVRKISM DYINLPVFNP
661NVFSEDEDDL PVTGASGVPE NSPPCTVNIP IAPIHSSAQA MSPTQSIGLV QSLLANQNVQ
721LDVLTNQTTA VGTAEHAGDS ATQYPVSNRY SNPGQVIFGS VEMGRIIQNP PPLSLPPPPQ
781GPMQLSTVGH GDRDHEHLQK SAKALRPTPQ LAAEGDAVVF SAPQEVQVTK INPPPPYPGT
841IPAAPTTAAP PPPLPPPQPP VDVCLKKGDF SLYPTSVHYQ TPLGYERITT FDSSGNVEEV
901CRPRTRMLCS QNTYTLPGPG SSATLRLTAT EKKVPQPCSS ATLNRLTVPR YSIPTGDPPP
961YPEIASQLAQ GRGAAQRSDN SLIHATLRRN NREATLKMAQ LADSPRAPLQ PLAKSKGGPG
1021GVVTQLPARP PPALYTCSQC SGTGPSSQPG ASLAHTASAS PLASQSSYSL LSPPDSARDR
1081TDYVNSAFTE DEALSQHCQL EKPLRHPPLP EAAVTLKRPP PYQWDPMLGE DVWVPQERTA
1141QTSGPNPLKL SSLMLSQGQH LDVSRLPFIS PKSPASPTAT FQTGYGMGVP YPGSYNNPPL
1201PGVQAPCSPK DALSPTQFAQ QEPAVVLQPL YPPSLSYCTL PPMYPGSSTC SSLQLPPVAL
1261HPWSSYSACP PMQNPQGTLP PKPHLVVEKP LVSPPPADLQ SHLGTEVMVE TADNFQEVLS
1321LTESPVPQRT EKFGKKNRKR LDSRAEEGSV QAITEGKVKK EARTLSDFNS LISSPHLGRE
1381KKKVKSQKDQ LKSKKLNKTN EFQDSSESEP ELFISGDELM NQSQGSRKGW KSKRSPRAAG
1441ELEEAKCRRA SEKEDGRLGS QGFVYVMANK QPLWNEATQV YQLDFGGRVT QESAKNFQIE
1501LEGRQVMQFG RIDGSAYILD FQYPFSAVQA FAVALANVTQ RLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.