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TULP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TULP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117421-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TULP2 (NM_003323), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 65 | Tubby like protein 2 | TUBL2 | TULP2 | Tubby-related protein 2 | Cancer testis antigen 65 | CT65 | Tubby-like protein 2
Origin Species
Human
NCBI GenBank
NM_003323
SwissProt
O00295

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSQDNDTLMR DILGHELAAM RLQKLEQQRR LFEKKQRQKR QELLMVQANP DASPWLWRSC
61LREERLLGDR GLGNPFLRKK VSEAHLPSGI HSALGTVSCG GDGRGERGLP TPRTEAVFRN
121LGLQSPFLSW LPDNSDAELE EVSVENGSVS PPPFKQSPRI RRKGWQAHQR PGTRAEGESD
181SQDMGDAHKS PNMGPNPGMD GDCVYENLAF QKEEDLEKKR EASESTGTNS SAAHNEELSK
241ALKGEGGTDS DHMRHEASLA IRSPCPGLEE DMEAYVLRPA LPGTMMQCYL TRDKHGVDKG
301LFPLYYLYLE TSDSLQRFLL AGRKRRRSKT SNYLISLDPT HLSRDGDNFV GKVRSNVFST
361KFTIFDNGVN PDREHLTRNT ARIRQELGAV CYEPNVLGYL GPRKMTVILP GTNSQNQRIN
421VQPLNEQESL LSRYQRGDKQ GLLLLHNKTP SWDKENGVYT LNFHGRVTRA SVKNFQIVDP
481KHQEHLVLQF GRVGPDTFTM DFCFPFSPLQ AFSICLSSFN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.