Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TUBGCP5 / GPC5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108102-100
Catalog Number: LS-G108102
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TUBGCP5 (NM_052903), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GCP-5 | KIAA1899 | TUBGCP5 | GCP5
- Origin Species
- Human
- NCBI GenBank
- AB067486
- SwissProt
- Q96RT8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 118.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARHGPPWSR LDAQQERDVR ELVRGVAGLQ DEADPNFQLA LNFAWSNFRF HRFLDVNSHK61IEKTIEGIYE KFVIHSDLSK AASWKRLTEE FLNAPLPSIK EIKTDAHYSI LSLLLCLSDS121PSNSSYVETP RNKEVEKKDD FDWGKYLMED EEMDIGPYMD TPNWSEESEE ENDQQPLSRE181DSGIQVDRTP LEEQDQNGKL DPCISWKDEP DDRSWLEHHV VHQYWTARPS QFPHSLHLHS241NLAAVWDQHL YSSDPLYVPD DRVLVTETQV IRETLWLLSG VKKLFIFQLI DGKVTVRNNI301IVTHLTHSCL RSVLEQIAAY GQVVFRLQEF IDEVMGHSSE SMLPGSGSVP KKSTEAPFRT361YQAFMWALYK YFISFKEELA EIEKCIINND TTITLAIVVD KLAPRLSQLK VLHKVFSTGV421AEVPPDTRNV VRASHLLNTL YKAILEYDNV GEASEQTVSL LFSLWVETVR PYLQTVDEWI481VHGHLWDGAR EFIIQRNKNV PVNHRDFWYA TYTLYSVSEK TENEEKMSDN ASASSGSDQG541PSSRQHTMVS FLKPVLKQII MAGKSMQLLK NLQCAESTTC QAGARDAERK SLYTLFLESV601QSRLRHGEDS TPQVLTEQQA TKENLMKMQS IAESHLELDD VHDPLLAINF ARMYLEQSDF661HEKFAGGDVC VDRSSESVTC QTFELTLRSC LYPHIDKQYL DCCGNLMQTL KKDYRLVEYL721QAMRNFFLME GGDTMYDFYT SIFDKIREKE TWQNVSFLNV QLQEAVGQRY PEDSSRLSIS781FENVDTAKKK LPVHILDGLT LSYKVPWPVD IVISLECQKI YNQVFLLLLQ IKWAKYSLDV841LLFGELVSTA EKPRLKEGLI HEQDTVAQFG PQKEPVRQQI HRMFLLRVKL MHFVNSLHNY901IMTRILHSTG LEFQHQVEEA KDLDQLIKIH YRYLSTIHDR CLLREKVSFV KEAIMKVLNL961ALMFADGWQA GLGTWRMESI EKMESDFKNC HMFLVTILNK AVCRGSFPHL ESLALSLMAG1021MEQSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1024 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.