LSBio
TUBGCP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TUBGCP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113935-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GCP4(TUBGCP4) (NM_014444), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
76P | HGCP4 | HGrip76 | GCP-4 | TUBGCP4 | GCP4 | H76p
Origin Species
Human
NCBI GenBank
NM_014444
SwissProt
Q9UGJ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIHELLLALS GYPGSIFTWN KRSGLQVSQD FPFLHPSETS VLNRLCRLGT DYIRFTEFIE
61QYTGHVQQQD HHPSQQGQGG LHGIYLRAFC TGLDSVLQPY RQALLDLEQE FLGDPHLSIS
121HVNYFLDQFQ LLFPSVMVVV EQIKSQKIHG CQILETVYKH SCGGLPPVRS ALEKILAVCH
181GVMYKQLSAW MLHGLLLDQH EEFFIKQGPS SGNVSAQPEE DEEDLGIGGL TGKQLRELQD
241LRLIEEENML APSLKQFSLR VEILPSYIPV RVAEKILFVG ESVQMFENQN VNLTRKGSIL
301KNQEDTFAAE LHRLKQQPLF SLVDFEQVVD RIRSTVAEHL WKLMVEESDL LGQLKIIKDF
361YLLGRGELFQ AFIDTAQHML KTPPTAVTEH DVNVAFQQSA HKVLLDDDNL LPLLHLTIEY
421HGKEHKDATQ AREGPSRETS PREAPASGWA ALGLSYKVQW PLHILFTPAV LEKYNVVFKY
481LLSVRRVQAE LQHCWALQMQ RKHLKSNQTD AIKWRLRNHM AFLVDNLQYY LQVDVLESQF
541SQLLHQINST RDFESIRLAH DHFLSNLLAQ SFILLKPVFH CLNEILDLCH SFCSLVSQNL
601GPLDERGAAQ LSILVKGFSR QSSLLFKILS SVRNHQINSD LAQLLLRLDY NKYYTQAGGT
661LGSFGMTRTR PLEQKLISEE DLAANDILDY KDDDDKV

697 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.