Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TUBE1 / Tubulin Epsilon Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112773-100
Catalog Number: LS-G112773
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human epsilon Tubulin(TUBE1) (NM_016262), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Epsilon-tubulin | TUBE1 | TUBE | Tubulin epsilon chain | DJ142L7.2 | Tubulin | epsilon 1
- Origin Species
- Human
- NCBI GenBank
- NM_016262
- SwissProt
- Q9UJT0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTQSVVVQVG QCGNQIGCCF WDLALREHAA VNQKGIYDEA ISSFFRNVDT RVVGDGGSIS61KGKICSLKAR AVLIDMEEGV VNEILQGPLR DVFDTKQLIT DISGSGNNWA VGHKVFGSLY121QDQILEKFRK SAEHCDCLQC FFIIHSMGGG TGSGLGTFLL KVLEDEFPEV YRFVTSIYPS181GEDDVITSPY NSILAMKELN EHADCVLPID NQSLFDIISK IDLMVNSGKL GTTVKPKSLV241TSSSGALKKQ HKKPFDAMNN IVANLLLNLT SSARFEGSLN MDLNEISMNL VPFPQLHYLV301SSLTPLYTLT DVNIPPRRLD QMFSDAFSKD HQLLRADPKH SLYLACALMV RGNVQISDLR361RNIERLKPSL QFVSWNQEGW KTSLCSVPPV GHSHSLLALA NNTCVKPTFM ELKERFMRLY421KKKAHLHHYL QVEGMEESCF TEAVSSLSAL IQEYDQLDAT KNMPVQDLPR LSIAMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
475 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.