LSBio
TUBB7P Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TUBB7P Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TUBB7P antibodies →
$725.00each
Catalog Number: LS-G111354-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TUBB4Q(TUBB4Q) (NM_020040), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
LOC388579 | TUBB4Q pseudogene | TUBB4Q
Origin Species
Human
NCBI GenBank
NM_020040
SwissProt
Q99867

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MRELVLTQTG QCGNQIGAKF WEVISDEHAI DSAGTYHGDS HLQLERINVH HHEASGGRYV
61SRAVLVDLEP GTMDSVRSGP FGQVFRPDNF ISRQCGAGNN WAKGRYTEGA ELTESVMDVV
121RKEAESCDCL QGFQLTHSLG GGTGSGMGTL LISKIWEEYP DRIINTLSIL LLPKVSDTVV
181EPYNATLSVH QLIENADETF CIDNEALYDI CSRTLKLPTP TYGDLNHLVS ATMSGVTTCL
241CFPDQLNADL RKLAMNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVAELT QQMFDAKNMM
301AARDPRHGRY LTAAAIFQGR MPMREVDEQM FNIQDKNSSY FADWFPNNVK TAVCDIPPWG
361LKMSVTFTGN NTAVQELKRV SEQFTATFRR KAFLHWYTGE GMDEMEFTEA ESNMNDLVSE
421YQQYQDATAE GGGV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

434 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.