Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TUBB6 / Tubulin Beta 6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108750-100
Catalog Number: LS-G108750
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TUBB6 (NM_032525), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Class V beta-tubulin | HsT1601 | TUBB-5 | TUBB6 | Tubulin beta MGC4083 | Tubulin beta-6 chain | Tubulin | beta 6 | beta 6 class V
- Origin Species
- Human
- NCBI GenBank
- NM_032525
- SwissProt
- Q9BUF5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MREIVHIQAG QCGNQIGTKF WEVISDEHGI DPAGGYVGDS ALQLERINVY YNESSSQKYV61PRAALVDLEP GTMDSVRSGP FGQLFRPDNF IFGQTGAGNN WAKGHYTEGA ELVDAVLDVV121RKECEHCDCL QGFQLTHSLG GGTGSGMGTL LISKIREEFP DRIMNTFSVM PSPKVSDTVV181EPYNATLSVH QLVENTDETY CIDNEALYDI CFRTLKLTTP TYGDLNHLVS ATMSGVTTSL241RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVPELT QQMFDARNMM301AACDPRHGRY LTVATVFRGP MSMKEVDEQM LAIQSKNSSY FVEWIPNNVK VAVCDIPPRG361LKMASTFIGN STAIQELFKR ISEQFSAMFR RKAFLHWFTG EGMDEMEFTE AESNMNDLVS421EYQQYQDATA NDGEEAFEDE EEEIDGTRTR PLEQKLISEE DLAANDILDY KDDDDKV
477 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.