Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TUBA3E / Tubulin Alpha 3E Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102872-100
Catalog Number: LS-G102872
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TUBA3E(TUBA3E) (NM_207312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha-tubulin 3E | Detyrosinated alpha Tubulin | Tubulin alpha-3E chain | Tubulin | alpha 3e | TUBA3E | TUBA2
- Origin Species
- Human
- SwissProt
- Q6PEY2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK61HVPRAVFVDL EPTVVDEVRT GTYRQLFHPE QLITGKEDAA SNYARGHYTI GKEIVDLVLD121RIRKLADLCT GLQGFLIFHS FGGGTGSGFA SLLMERLSVD YSKKSKLEFA IYPAPQVSTA181VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA241SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN301QMVKCDPRHG KYMACCMLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP361TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LVHKFDLMYA KWAFVHWYVG EGMEEGEFSE421AREDLAALEK DCEEVGVDSV EAEAEEGEEYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
450 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.