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TTLL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTLL6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTLL6 antibodies →
$504.00each
Catalog Number: LS-G105246-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTLL6 (NM_173623), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001130918

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGCLGVAVL RKLSTFSAYL EDHSYNVEQI WRDIEDVIIK TLISAHPIIR HNYHTCFPNH
61TLNSACFEIL GFDILLDHKL KPWLLEVNHS PSFSTDSRLD KEVKDGLLYD TLVLINLESC
121DKKKVLEEER QRGQFLQQCC SREMRIEEAK GFRAVQLKKT ETYEKENCGG FRLIYPSLNS
181EKYEKFFQDN NSLFQNTVAS RAREEYARQL IQELRLKREK KPFQMKKKVE MQGESAGEQV
241RKKGMRGWQQ KQQQKDKAAT QASKQYIQPL TLVSYTPDLL LSVRGERKNE TDSSLNQEAP
301TEEASSVFPK LTSAKPFSSL PDLRNINLSS SKLEPSKPNF SIKEAKSASA VNVFTGTVHL
361TSVETTPEST TQLSISPKSP PTLAVTASSE YSGPETDRVV SFKCKKQQTP PHLTQKKMLK
421SFLPTKSKSF WESPNTNWTL LKSDMNKPHL ISELLTKLQL SGKLSFFPAH YNPKLGMNNL
481SQNPSLPGEC HSRSDSSGEK RQLDVSSLLL QSPQSYNVTL RDLLVIATPA QLDPRPCRSH
541ASAMRDPCMQ DQEAYSHCLI SGQKGCERS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

569 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.