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TTLL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTLL4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTLL4 antibodies →
$504.00each
Catalog Number: LS-G113810-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTLL4 (NM_014640), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0173 | TTLL4 | Tubulin polyglutamylase TTLL4
Origin Species
Human
NCBI GenBank
NM_014640
SwissProt
Q14679

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASAGTQHYS IGLRQKSSFK QSGPSGTVPA TPPEKPSEGR VWPQAHQQVK PIWKLEKKQV
61ETLSAGLGPG LLGVPPQPAY FFCPSTLCSS GTTAVIAGHS SSCYLHSLPD LFNSTLLYRR
121SSYRQKPYQQ LESFCLRSSP SEKSPFSLPQ KSLPVSLTAN KATSSMVFSM AQPMASSSTE
181PYLCLAAAGE NPSGKSLASA ISGKIPSPLS SSYKPMLNNN SFMWPNSTPV PLLQTTQGLK
241PVSPPKIQPV SWHHSGGTGD CAPQPVDHKV PKSIGTVPAD ASAHIALSTA SSHDTSTTSV
301ASSWYNRNNL AMRAEPLSCA LDDSSDSQDP TKEIRFTEAV RKLTARGFEK MPRQGCQLEQ
361SSFLNPSFQW NVLNRSRRWK PPAVNQQFPQ EDAGSVRRVL PGASDTLGLD NTVFCTKRIS
421IHLLASHASG LNHNPACESV IDSSAFGEGK APGPPFPQTL GIANVATRLS SIQLGQSEKE
481RPEEARELDS SDRDISSATD LQPDQAETED TEEELVDSLE DCCGRDENEE EEGDSECSSL
541SAVSPSESVA MISRSCMEIL TKPLSNHEKV VRPALIYSLF PNVPPTIYFG TRDERVEKLP
601WEQRKLLRWK MSTVTPNIVK QTIGRSHFKI SKRNDDWLGC WGHHMKSPSF RSIREHQKLN
661HFPGSFQIGR KDRLWRNLSR MQSRFGKKEF SFFPQSFILP QDAKLLRKAW ESSSRQKWIV
721KPPASARGIG IQVIHKWSQL PKRRPLLVQR YLHKPYLISG SKFDLRIYVY VTSYDPLRIY
781LFSDGLVRFA SCKYSPSMKS LGNKFMHLTN YSVNKKNAEY QANADEMACQ GHKWALKALW
841NYLSQKGVNS DSIWEKIKDV VVKTIISSEP YVTSLLKMYV RRPYSCHELF GFDIMLDENL
901KPWVLEVNIS PSLHSSSPLD ISIKGQMIRD LLNLAGFVLP NAEDIISSPS SCSSSTTSLP
961TSPGDKCRMA PEHVTAQKMK KAYYLTQKIP DQDFYASVLD VLTPDDVRIL VEMEDEFSRR
1021GQFERIFPSH ISSRYLRFFE QPRYFNILTT QWEQKYHGNK LKGVDLLRSW CYKGFHMGVV
1081SDSAPVWSLP TSLLTISKDD VILNAFSKSE TSKLGKQSSC EVSLLLSEDG TTPKSKKTQA
1141GLSPYPQKPS SSKDSEDTSK EPSLSTQTLP VIKCSGQTSR LSASSTFQSI SDSLLAVSP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1199 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.