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TTLL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTLL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTLL3 antibodies →
$725.00each
Catalog Number: LS-G121005-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTLL3 (NM_001025930), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TTLL3 | Tubulin monoglycylase TTLL3 | DKFZP434B103 | HOTTL
Origin Species
Human
NCBI GenBank
NM_015644

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.62 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQGPDAPLLL SARELGPGRR GSASWYRQEG GAVCNWLRKP QPLEPRTSFP SARRSEFRPP
61RRLPWAGPAS AQSEEGHAGG RCQAGSPAPA RRLSGAFKTL VPGTHAQGAS DTHPLGPPHT
121PVLDPSPQDG FPVLWRGSSK ASHMNRLRNA KIYVERAVKQ KKIFTIQGCY PVIRCLLRRR
181GWVEKKMVHR SGPTLLPPQK DLDSSAMGDS DTTEDEDEDE DEEFQPSQLF DFDDLLKFDD
241LDGTHALMVG LCLNLRNLPW FDEVDANSFF PRCYCLGAED DKKAFIEDFW LTAARNVLKL
301VVKSEWKSYP IQAVEEEASG DKQPKKQEKN PVLVSPEFVD EALCACEEYL SNLAHMDIDK
361DLEAPLYLTP EGWSLFLQRY YQVVHEGAEL RHLDTQVQRC EDILQQLQAV VPQIDMEGDR
421NIWIVKPGAK SRGRGIMCMD HLEEMLKLVN GNPVVMKDGK WVVQKYIERP LLIFGTKFDL
481RQWFLVTDWN PLTVWFYRDS YIRFSTQPFS LKNLDNSVHL CNNSIQKHLE NSCHRHPLLP
541PDNMWSSQRF QAHLQEMGAP NAWSTIIVPG MKDAVIHALQ TSQDTVQCRK ASFELYGADF
601VFGEDFQPWL IEINASPTMA PSTAVTARLC AGVQADTLRV VIDRMLDRNC DTGAFELIYK
661QPAVEVPQYV GIRLLVEGFT IKKPMAMCHR RMGVRPAVPL LTQRGSGEAR HHFPSLHTKA
721QLPSPHVLRH QGQVLRRQHS KLVGTKALST TGKALRTLPT AKVFISLPPN LDFKVAPSIL
781KPRKAPALLC LRGPQLEVPC CLCPLKSEQF LAPVGRSRPK ANSRPDCDKP RAEACPMKRL
841SPLKPLPLVG TFQRRRGLGD MKLGKPLLRF PTALVLDPTP NKKKQVKYLG LDSIAVGGSR
901VDGARPCTPG STARA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

915 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.