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TTLL11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTLL11 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTLL11 antibodies →
$725.00each
Catalog Number: LS-G125795-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTLL11 (NM_001139442), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA244O19.1 | C9orf20 | TTLL11 | RP11-429D3.1 | Tubulin polyglutamylase TTLL11
Origin Species
Human
NCBI GenBank
NM_001139442
SwissProt
Q8NHH1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAASVTGR VTWAASPMRS LGLGRRLSLP GPRLDAVTAA VNPSLSDHGN GLGRGTRGSG
61CSGGSLVADW GGGAAAAAAV ALALAPALST MRRGSSESEL AARWEAEAVA AAKAAAKAEA
121EATAETVAEQ VRVDAGAAGE PECKAGEEQP KVLAPAPAQP SAAEEGNTQV LQRPPPTLPP
181SKPKPVQGLC PHGKPRDKGR SCKRSSGHGS GENGSQRPVT VDSSKARTSL DALKISIRQL
241KWKEFPFGRR LPCDIYWHGV SFHDNDIFSG QVNKFPGMTE MVRKITLSRA VRTMQNLFPE
301EYNFYPRSWI LPDEFQLFVA QVQMVKDDDP SWKPTFIVKP DGGCQGDGIY LIKDPSDIRL
361AGTLQSRPAV VQEYICKPLL IDKLKFDIRL YVLLKSLDPL EIYIAKDGLS RFCTEPYQEP
421TPKNLHRIFM HLTNYSLNIH SGNFIHSDSA STGSKRTFSS ILCRLSSKGV DIKKVWSDII
481SVVIKTVIAL TPELKVFYQS DIPTGRPGPT CFQILGFDIL LMKNLKPILL EVNANPSMRI
541EHEHELSPGV FENVPSLVDE EVKVAVIRDT LRLMDPLKKK RENQSQQLEK PFAGKEDALD
601GELTSAPDCN ANPEAHLPSI CLKQVFPKYA KQFNYLRLVD RMANLFIRFL GIKGTMKLGP
661TGFRTFIRSC KLSSSSLSMA AVDILYIDIT RRWNSMTLDQ RDSGMCLQAF VEAFFFLAQR
721KFKMLPLHEQ VASLIDLCEY HLSLLDEKRL VCGRGVPSGG RPPHRGPPQE PSPSAQPAGD
781NPPPRTSCAN KLSHPRHTLS
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

800 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.