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TTLL10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTLL10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTLL10 antibodies →
$725.00each
Catalog Number: LS-G124972-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTLL10 (NM_001130045), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Inactive polyglycylase TTLL10 | RP5-902P8.1 | TTLL10
Origin Species
Human
NCBI GenBank
NM_001130045
SwissProt
Q6ZVT0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDHSCTRFIH RRGPPTRTRA GFKRGKRPRI QQRPRARVSG TIPASRLHPA PASQPGPCPA
61PGHCPVGPAH ERPMGSSQEE GLRCQPSQPD HDADGHCGPD LEGAERASAT PGPPGLLNSH
121RPADSDDTNA AGPSAALLEG LLLGGGKPSP HSTRPGPFFY IGGSNGATII SSYCKSKGWQ
181RIHDSRRDDY TLKWCEVKSR DSYGSFREGE QLLYQLPNNK LLTTKIGLLS TLRGRARAMS
241KASKVPGGVQ ARLEKDAAAP ALEDLPWTSP GYLRPQRVLR MEEFFPETYR LDLKHEREAF
301FTLFDETQIW ICKPTASNQG KGIFLLRNQE EVAALQAKTR SMEDDPIHHK TPFRGPQARV
361VQRYIQNPLL VDGRKFDVRS YLLIACTTPY MIFFGHGYAR LTLSLYDPHS SDLGGHLTNQ
421FMQKKSPLYM LLKEHTVWSM EHLNRYISDT FWKARGLAKD WVFTTLKKRM QQIMAHCFLA
481AKPKLDCKLG YFDLIGCDFL IDDNFKVWLL EMNSNPALHT NCEVLKEVIP GVVIETLDLV
541LETFRKSLRG QKMLPLLSQR RFVLLHNGEA DPRPHLGGSC SLRRWPPLPT RQAKSSGPPM
601PHAPDQPGAR RPAPPPLVPQ RPRPPGPDLD SAHDGEPQAP GTEQSGTGNR HPAQEPSPGT
661AKEEREEPEN ARP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

673 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.