Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TTI2 / C8orf41 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109585-100
Catalog Number: LS-G109585
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C8ORF41(TTI2) (NM_025115), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C8orf41 | TTI2 | TELO2-interacting protein 2 | TELO2 interacting protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_025115
- SwissProt
- Q6NXR4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELDSALEAP SQEDSNLSEE LSHSAFGQAF SKILHCLARP EARRGNVKDA VLKDLGDLIE61ATEFDRLFEG TGARLRGMPE TLGQVAKALE KYAAPSKEEE GGGDGHSEAA EKAAQVGLLF121LKLLGKVETA KNSLVGPAWQ TGLHHLAGPV YIFAITHSLE QPWTTPRSRE VAREVLTSLL181QVTECGSVAG FLHGENEDEK GRLSVILGLL KPDLYKESWK NNPAIKHVFS WTLQQVTRPW241LSQHLERVLP ASLVISDDYQ TENKILGVHC LHHIVLNVPA ADLLQYNRAQ VLYHAISNHL301YTPEHHLIQA VLLCLLDLFP ILEKTLHWKG DGARPTTHCD EVLRLILTHM EPEHRLLLRR361TYARNLPAFV NRLGILTVRH LKRLERVIIG YLEVYDGPEE EARLKILETL KLLMQHTWPR421VSCRLVVLLK ALLKLICDVA RDPNLTPESV KSALLQEATD CLILLDRCSQ GRVKGLLAKI481PQSCEDRKVV NYIRKVQQVS EGAPYNGTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
508 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.