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TTI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTI1 antibodies →
$504.00each
Catalog Number: LS-G101246-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTI1 (NM_014657), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0406 | Protein SMG10 | SMG10 | TELO2 interacting protein 1 | TTI1 | Smg-10 | Tel two interacting protein 1
Origin Species
Human
NCBI GenBank
NM_014657

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
121.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVFDTPEEA FGVLRPVCVQ LTKTQTVENV EHLQTRLQAV SDSALQELQQ YILFPLRFTL
61KTPGPKRERL IQSVVECLTF VLSSTCVKEQ ELLQELFSEL SACLYSPSSQ KPAAVSEELK
121LAVIQGLSTL MHSAYGDIIL TFYEPSILPR LGFAVSLLLG LAEQEKSKQI KIAALKCLQV
181LLLQCDCQDH PRSLDELEQK QLGDLFASFL PGISTALTRL ITGDFKQGHS IVVSSLKIFY
241KTVSFIMADE QLKRISKVQA KPAVEHRVAE LMVYREADWV KKTGDKLTIL IKKIIECVSV
301HPHWKVRLEL VELVEDLLLK CSQSLVECAG PLLKALVGLV NDESPEIQAQ CNKVLRHFAD
361QKVVVGNKAL ADILSESLHS LATSLPRLMN SQDDQGKFST LSLLLGYLKL LGPKINFVLN
421SVAHLQRLSK ALIQVLELDV ADIKIVEERR WNSDDLNASP KTSATQPWNR IQRRYFRFFT
481DERIFMLLRQ VCQLLGYYGN LYLLVDHFME LYHQSVVYRK QAAMILNELV TGAAGLEVED
541LHEKHIKTNP EELREIVTSI LEEYTSQENW YLVTCLETEE MGEELMMEHP GLQAITSGEH
601TCQVTSFLAF SKPSPTICSM NSNIWQICIQ LEGIGQFAYA LGKDFCLLLM SALYPVLEKA
661GDQTLLISQV ATSTMMDVCR ACGYDSLQHL INQNSDYLVN GISLNLRHLA LHPHTPKVLE
721VMLRNSDANL LPLVADVVQD VLATLDQFYD KRAASFVSVL HALMAALAQW FPDTGNLGHL
781QEQSLGEEGS HLNQRPAALE KSTTTAEDIE QFLLNYLKEK DVADGNVSDF DNEEEEQSVP
841PKVDENDTRP DVEPPLPLQI QIAMDVMERC IHLLSDKNLQ IRLKVLDVLD LCVVVLQSHK
901NQLLPLAHQA WPSLVHRLTR DAPLAVLRAF KVLRTLGSKC GDFLRSRFCK DVLPKLAGSL
961VTQAPISARA GPVYSHTLAF KLQLAVLQGL GPLCERLDLG EGDLNKVADA CLIYLSVKQP
1021VKLQEAARSV FLHLMKVDPD STWFLLNELY CPVQFTPPHP SLHPVQLHGA SGQQNPYTTN
1081VLQLLKELQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1089 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.