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TTC8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC8 antibodies →
$504.00each
Catalog Number: LS-G103778-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC8 (NM_198309), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bardet-Biedl syndrome type 8 | BBS8 | RP51 | TPR repeat protein 8 | TTC8
Origin Species
Human
NCBI GenBank
W37688
SwissProt
Q8TAM2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MSSEMEPLLL AWSYFRRRKF QLCADLCTQM LEKSPYDQAA WILKARALTE MVYIDEIDVD
61QEGIAEMMLD ENAIAQVPRP GTSLKLPGTN QTGGPSQAVR PITQAGRPIT GFLRPSTQSG
121RPGTMEQAIR TPRTAYTARP ITSSSGRFVR LGTASMLTSP DGPFINLSRL NLTKYSQKPK
181LAKALFEYIF HHENDVKTAL DLAALSTEHS QYKDWWWKVQ IGKCYYRLGM YREAEKQFKS
241ALKQQEMVDT FLYLAKVYVS LDQPVTALNL FKQGLDKFPG EVTLLCGIAR IYEEMNNMSS
301AAEYYKEVLK QDNTHVEAIA CIGSNHFYSD QPEIALRFYR RLLQMGIYNG QLFNNLGLCC
361FYAQQYDMTL TSFERALSLA ENEEEAADVW YNLGHVAVGI GDTNLAHQCF RLALVNNNNH
421AEAYNNLAVL EMRKGHVEQA RALLQTASSL APHMYEPHFN FATISDKIGD LQRSYVAAQK
481SEAAFPDHVD TQHLIKQLRQ HFAML
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

505 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.