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TTC7A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC7A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC7A antibodies →
$725.00each
Catalog Number: LS-G111146-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC7A (NM_020458), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1140 | TTC7A | TPR repeat protein 7A | TTC7
Origin Species
Human
NCBI GenBank
AB032966
SwissProt
Q9ULT0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAKGAHGSY LKVESELERC RAEGHWDRMP ELVRQLQTLS MPGGGGNRRG SPSAAFTFPD
61TDDFGKLLLA EALLEQCLKE NHAKIKDSMP LLEKNEPKMS EAKNYLSSIL NHGRLSPQYM
121CEAMLILGKL HYVEGSYRDA ISMYARAGID DMSMENKPLY QMRLLSEAFV IKGLSLERLP
181NSIASRFRLT EREEEVITCF ERASWIAQVF LQELEKTTNN STSRHLKGCH PLDYELTYFL
241EAALQSAYVK NLKKGNIVKG MRELREVLRT VETKATQNFK VMAAKHLAGV LLHSLSEECY
301WSPLSHPLPE FMGKEESSFA TQALRKPHLY EGDNLYCPKD NIEEALLLLL ISESMATRDV
361VLSRVPEQEE DRTVSLQNAA AIYDLLSITL GRRGQYVMLS ECLERAMKFA FGEFHLWYQV
421ALSMVACGKS AYAVSLLREC VKLRPSDPTV PLMAAKVCIG SLRWLEEAEH FAMMVISLGE
481EAGEFLPKGY LALGLTYSLQ ATDATLKSKQ DELHRKALQT LERAQQLAPS DPQVILYVSL
541QLALVRQISS AMEQLQEALK VRKDDAHALH LLALLFSAQK HHQHALDVVN MAITEHPENF
601NLMFTKVKLE QVLKGPEEAL VTCRQVLRLW QTLYSFSQLG GLEKDGSFGE GLTMKKQSGM
661HLTLPDAHDA DSGSRRASSI AASRLEEAMS ELTMPSSVLK QGPMQLWTTL EQIWLQAAEL
721FMEQQHLKEA GFCIQEAAGL FPTSHSVLYM RGRLAEVKGN LEEAKQLYKE ALTVNPDGVR
781IMHSLGLMLS RLGHKSLAQK VLRDAVERQS TCHEAWQGLG EVLQAQGQNE AAVDCFLTAL
841ELEASSPVLP FSIIPREL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

858 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.