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TTC30B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC30B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC30B antibodies →
$504.00each
Catalog Number: LS-G106199-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC30B (NM_152517), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IFT70 | Fleer | TTC30B | TPR repeat protein 30B
Origin Species
Human
SwissProt
Q8N4P2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGLSGAQIP DGEFTAVVYR LIRNARYAEA VQLLGGELQR SPRSRAGLSL LGYCYYRLQE
61FALAAECYEQ LGQLHPELEQ YRLYQAQALY KACLYAEATR VAFLLLDNPA YHSRVLHLQA
121AIKYSEGDLP GSRSLVEQLP SREGGEESGG ENETDGQINL GCLLYKEGQY EAACSKFFAA
181LQASGYQPDL SYNLALAYYS SRQYASALKH IAEIIERGIR QHPELGVGMT TEGIDVRSVG
241NTLVLHQTAL VEAFNLKAAI EYQLRNYEAA QEALTDMPPR AEEELDPVTL HNQALMNMDA
301RPTEGFEKLQ FLLQQNPFPP ETFGNLLLLY CKYEYFDLAA DVLAENAHLI YKFLTPYLYD
361FLDAVITCQT APEEAFIKLD GLAGMLTEVL RKLTIQVQEA RHNRDDEAIK KAVNEYDETM
421EKYIPVLMAQ AKIYWNLENY PMVEKIFRKS VEFCNDHDVW KLNVAHVLFM QENKYKEAIG
481FYEPIVKKHY DNILNVSAIV LANLCVSYIM TSQNEEAEEL MRKIEKEEEQ LSYDDPDKKM
541YHLCIVNLVI GTLYCAKGNY DFGISRVIKS LEPYNKKLGT DTWYYAKRCF LSLLENMSKH
601TIMLRDSVIQ ECVQFLEHCE LHGRNIPAVI EQPLEEERMH VGKNTVTYES RQLKALIYEI
661IGWNI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

665 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.