LSBio
TTC27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC27 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC27 antibodies →
$504.00each
Catalog Number: LS-G112260-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC27 (NM_017735), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TPR repeat protein 27 | TTC27
Origin Species
Human
NCBI GenBank
NM_017735
SwissProt
Q6P3X3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MWTPELAILR GFPTEAERQQ WKQEGVVGSE SGSFLQLLLE GNYEAIFLNS MTQNIFNSTT
61TAEEKIDSYL EKQVVTFLDY STDLDTTERQ QLIFLLGVSS LQLFVQSNWT GPPVDLHPQD
121FLSSVLFQQF SEVKGLDAFV LSLLTLDGES IYSLTSKPIL LLLARIILVN VRHKLTAIQS
181LPWWTLRCVN IHQHLLEERS PLLFTLAENC IDQVMKLQNL FVDDSGRYLA IQFHLECAYV
241FLYYYEYRKA KDQLDIAKDI SQLQIDLTGA LGKRTRFQEN YVAQLILDVR REGDVLSNCE
301FTPAPTPQEH LTKNLELNDD TILNDIKLAD CEQFQMPDLC AEEIAIILGI CTNFQKNNPV
361HTLTEVELLA FTSCLLSQPK FWAIQTSALI LRTKLEKGST RRVERAMRQT QALADQFEDK
421TTSVLERLKI FYCCQVPPHW AIQRQLASLL FELGCTSSAL QIFEKLEMWE DVVICYERAG
481QHGKAEEILR QELEKKETPS LYCLLGDVLG DHSCYDKAWE LSRYRSARAQ RSKALLHLRN
541KEFQECVECF ERSVKINPMQ LGVWFSLGCA YLALEDYQGS AKAFQRCVTL EPDNAEAWNN
601LSTSYIRLKQ KVKAFRTLQE ALKCNYEHWQ IWENYILTST DVGEFSEAIK AYHRLLDLRD
661KYKDVQVLKI LVRAVIDGMT DRSGDVATGL KGKLQELFGR VTSRVTNDGE IWRLYAHVYG
721NGQSEKPDEN EKAFQCLSKA YKCDTQSNCW EKDITSFKEV VQRALGLAHV AIKCSKNKSS
781SQEAVQMLSS VRLNLRGLLS KAKQLFTDVA TGEMSRELAD DITAMDTLVT ELQDLSNQFR
841NQY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

843 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.