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TTC26 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC26 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC26 antibodies →
$504.00each
Catalog Number: LS-G126412-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC26 (NM_001144923), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dyf-13 | DYF13 | TPR repeat protein 26 | TTC26
Origin Species
Human
NCBI GenBank
NM_024926
SwissProt
A0AVF1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MMLSRAKPAV GRGVQHTDKR KKKGRKIPKL EELLSKRDFT GAITLLEEYE NATKEENCNS
61EVWVNLACTY FFLGMYKQAE AAGFKASKSR LQNRLLFHLA HKFNDEKKLM SFHQNLQDVT
121EDQLSLASIH YMRSHYQEAI DIYKRILLDN REYLALNVYV ALCYYKLDYY DVSQEVLAVY
181LQQIPDSTIA LNLKACNHFR LYNGRAAEAE LKSLMDNASS SFEFAKELIR HNLVVFRGGE
241GALQVLPPLV DVIPEARLNL VIYYLRQDDV QEAYNLIKDL EPTTPQEYIL KGVVNAALGQ
301EMGSRDHMKI AQQFFQLVGG SASECDTIPG RQCMASCFFL LKQFDDVLIY LNSFKSYFYN
361DDIFNFNYAQ AKAATGNTSE GEEAFLLIQS EKMKNDYIYL SWLARCYIMN KKPRLAWELY
421LKMETSGESF SLLQLIANDC YKMGQFYYSA KAFDVLERLD PNPEYWEGKR GACVGIFQMI
481IAGREPKETL REVLHLLRST GNTQVEYMIR IMKKWAKENR VSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

523 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.