LSBio
TTC25 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC25 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC25 antibodies →
$504.00each
Catalog Number: LS-G109225-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC25 (NM_031421), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434H0115 | TPR repeat protein 25 | TTC25
Origin Species
Human
NCBI GenBank
NM_031421
SwissProt
Q96NG3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDPEGETLR STFPSYMAEG ERLYLCGEFS KAAQSFSNAL YLQDGDKNCL VARSKCFLKM
61GDLERSLKDA EASLQSDPAF CKGILQKAET LYTMGDFEFA LVFYHRGYKL RPDREFRVGI
121QKAQEAINNS VGSPSSIKLE NKGDLSFLSK QAENIKAQQK PQPMKHLLHP TKGEPKWKAS
181LKSEKTVRQL LGELYVDKEY LEKLLLDEDL IKGTMKGGLT VEDLIMTGIN YLDTHSNFWR
241QQKPIYARER DRKLMQEKWL RDHKRRPSQT AHYILKSLED IDMLLTSGSA EGSLQKAEKV
301LKKVLEWNKE EVPNKDELVG NLYSCIGNAQ IELGQMEAAL QSHRKDLEIA KEYDLPDAKS
361RALDNIGRVF ARVGKFQQAI DTWEEKIPLA KTTLEKTWLF HEIGRCYLEL DQAWQAQNYG
421EKSQQCAEEE GDIEWQLNAS VLVAQAQVKL RDFESAVNNF EKALERAKLV HNNEAQQAII
481SALDDANKGI IRELRKTNYV ENLKEKSEGE ASLYEDRIIT REKDMRRVRD EPEKVVKQWD
541HSEDEKETDE DDEAFGEALQ SPASGKQSVE AGKARSDLGA VAKGLSGELG TRSGETGRKL
601LEAGRRESRE IYRRPSGELE QRLSGEFSRQ EPEELKKLSE VGRREPEELG KTQFGEIGET
661KKNRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

665 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.