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TTC22 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC22 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC22 antibodies →
$504.00each
Catalog Number: LS-G124326-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC22 (NM_001114108), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TPR repeat protein 22 | TTC22
Origin Species
Human
NCBI GenBank
NM_017904
SwissProt
Q5TAA0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELEAVADD LDALIDDLDY LPGHFHLEMQ LNFEPRSPAP QRARDLKLQR EGLRQELQLA
61AAPQRPAVRH LLGAFAFYLE ELDEARECFL EVAHEHPGNL NAWANLAHVY GRLGQEEEEE
121ACAARLADLM GLAEEPEAAG DPQLRAARCL AEQGYAHGFD VGCASPEERA RGLAAGIALY
181DKALGYGQQI PMEEKRGWYF TMATLYIRLD GIFLELGSEE QKRLPAFNRT LALLRQVLKS
241EDPRHRALAW CYLGMLLERK DTFSTTPMGV HDCGYSGTDP LDCFGKAIEI AKNQPPILNR
301LAKIFYFLGK QDMAIGTCNM ALDVLRDPEL NWQAYCTRAK IHIRAYLHDL KRAKMGLGGM
361PDRNHLACAK ADLEEVVRVC PGFKAYLDIG QVYYYMGVDA VQELLAVDEA ALNQALVFLA
421KAGESELGAT LPELQLLRGK CLRIKGEDAN AAACFKRAVE LDDAGSSHTD GFGCLLEALL
481AQWSQAQLSD GELGREVDAW LRRAQDKYPA ARLRQELQRV WRGHTDEVLG LARALVAQGR
541PALVRLLFET MEREGEGASA PRDRRAVSF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

569 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.