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TTC21B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC21B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC21B antibodies →
$504.00each
Catalog Number: LS-G109793-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC21B (NM_024753), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATD4 | Nbla10696 | NPHP12 | THM1 | TPR repeat protein 21B | IFT139 | JBTS11 | KIAA1992 | TTC21B
Origin Species
Human
NCBI GenBank
NM_024753
SwissProt
Q7Z4L5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
150.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSQELKTLI NYYCQERYFH HVLLVASEGI KRYGSDPVFR FYHAYGTLME GKTQEALREF
61EAIKNKQDVS LCSLLALIYA HKMSPNPDRE AILESDARVK EQRKGAGEKA LYHAGLFLWH
121IGRHDKAREY IDRMIKISDG SKQGHVLKAW LDITRGKEPY TKKALKYFEE GLQDGNDTFA
181LLGKAQCLEM RQNYSGALET MNQIIVNFPS FLPAFVKKMK LQLALQDWDQ TVETAQRLLL
241QDSQNVEALR MQALYYVCRE GDIEKASTKL ENLGNALDAM EPQNAQLFYN ITLAFSRTCG
301RSQLILQKIQ TLLERAFSLN PQQSEFATEL GYQMILQGRV KEALKWYKTA MTLDETSVSA
361LVGFIQCQLI EGQLQDADQQ LEFLNEIQQS IGKSAELIYL HAVLAMKKNK RQEEVINLLN
421DVLDTHFSQL EGLPLGIQYF EKLNPDFLLE IVMEYLSFCP MQPASPGQPL CPFLRRCISV
481LETVVRTVPG LLQTVFLIAK VKYLSGDIEA AFNNLQHCLE HNPSYADAHL LLAQVYLSQE
541KVKLCSQSLE LCLSYDFKVR DYPLYHLIKA QSQKKMGEIA DAIKTLHMAM SLPGMKRIGA
601STKSKDRKTE VDTSHRLSIF LELIDVHRLN GEQHEATKVL QDAIHEFSGT SEEVRVTIAN
661ADLALAQGDI ERALSILQNV TAEQPYFIEA REKMADIYLK HRKDKMLYIT CFREIAERMA
721NPRSFLLLGD AYMNILEPEE AIVAYEQALN QNPKDGTLAS KMGKALIKTH NYSMAITYYE
781AALKTGQKNY LCYDLAELLL KLKWYDKAEK VLQHALAHEP VNELSALMED GRCQVLLAKV
841YSKMEKLGDA ITALQQAREL QARVLKRVQM EQPDAVPAQK HLAAEICAEI AKHSVAQRDY
901EKAIKFYREA LVHCETDNKI MLELARLYLA QDDPDSCLRQ CALLLQSDQD NEAATMMMAD
961LMFRKQDYEQ AVFHLQQLLE RKPDNYMTLS RLIDLLRRCG KLEDVPRFFS MAEKRNSRAK
1021LEPGFQYCKG LYLWYTGEPN DALRHFNKAR KDRDWGQNAL YNMIEICLNP DNETVGGEVF
1081ENLDGDLGNS TEKQESVQLA VRTAEKLLKE LKPQTVQGHV QLRIMENYCL MATKQKSNVE
1141QALNTFTEIA ASEKEHIPAL LGMATAYMIL KQTPRARNQL KRIAKMNWNA IDAEEFEKSW
1201LLLADIYIQS AKYDMAEDLL KRCLRHNRSC CKAYEYMGYI MEKEQAYTDA ALNYEMAWKY
1261SNRTNPAVGY KLAFNYLKAK RYVDSIDICH QVLEAHPTYP KIRKDILDKA RASLRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1316 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.