LSBio
TTC21A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC21A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC21A antibodies →
$725.00each
Catalog Number: LS-G124121-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC21A (NM_001105513), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001105513

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
151 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSNDSSLMA GIIYYSQEKY FHHVQQAAAV GLEKFSNDPV LKFFKAYGVL KEEHIQDAIS
61DLESIRHHPD VSLCSTMALI YAHKRCEIID REAIQELEYS LKEIRKTVSG TALYYAGLFL
121WLIGRHDKAK EYIDRMLKIS RGFREAYVLR GWVDLTSDKP HTAKKAIEYL EQGIQDTKDV
181LGLMGKAMYF MMQQNYSEAL EVVNQITVTS GSFLPALVLK MQLFLARQDW EQTVEMGHRI
241LEKDESNIDA CQILTVHELA REGNMTTVSS LKTQKATNHV RNLIKALETR EPENPSLHLK
301KIIVVSRLCG SHQVILGLVC SFIERTFMAT PSYVHVATEL GYLFILKNQV KEALLWYSEA
361MKLDKDGMAG LTGIILCHIL EGHLEEAEYR LEFLKEVQKS LGKSEVLIFL QALLMSRKHK
421GEEETTALLK EAVELHFSSM QGIPLGSEYF EKLDPYFLVC IAKEYLLFCP KQPRLPGQIV
481SPLLKQVAVI LNPVVKAAPA LIDPLYLMAQ VRYYSGELEN AQSILQRCLE LDPASVDAHL
541LMCQIYLAQG NFGMCFHCLE LGVSHNFQVR DHPLYHLIKA RALNKAGDYP EAIKTLKMVI
601KLPALKKEEG RKFLRPSVQP SQRASILLEL VEALRLNGEL HEATKVMQDT INEFGGTPEE
661NRITIANVDL VLSKGNVDVA LNMLRNILPK QSCYMEAREK MANIYLQTLR DRRLYIRCYR
721ELCEHLPGPH TSLLLGDALM SILEPEKALE VYDEAYRQNP HDASLASRIG HAYVKAHQYT
781EAIEYYEAAQ KINGQDFLCC DLGKLLLKLK KVNKAEKVLK QALEHDIVQD IPSMMNDVKC
841LLLLAKVYKS HKKEAVIETL NKALDLQSRI LKRVPLEQPE MIPSQKQLAA SICIQFAEHY
901LAEKEYDKAV QSYKDVFSYL PTDNKVMLEL AQLYLLQGHL DLCEQHCAIL LQTEQNHETA
961SVLMADLMFR KQKHEAAINL YHQVLEKAPD NFLVLHKLID LLRRSGKLED IPAFFELAKK
1021VSSRVPLEPG FNYCRGIYCW HIGQPNEALK FLNKARKDST WGQSAIYHMV QICLNPDNEV
1081VGGEAFENQG AESNYMEKKE LEQQGVSTAE KLLREFYPHS DSSQTQLRLL QGLCRLATRE
1141KANMEAALGS FIQIAQAEKD SVPALLALAQ AYVFLKQIPK ARMQLKRLAK TPWVLSEAED
1201LEKSWLLLAD IYCQGSKFDL ALELLRRCVQ YNKSCYKAYE YMGFIMEKEQ SYKDAVTNYK
1261LAWKYSHHAN PAIGFKLAFN YLKDKKFVEA IEICNDVLRE HPDYPKIREE ILEKARRSLR
1321P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1321 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.