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TTC19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC19 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC19 antibodies →
$504.00each
Catalog Number: LS-G112222-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC19 (NM_017775), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TPR repeat protein 19 | TTC19
Origin Species
Human
NCBI GenBank
NM_017775
SwissProt
Q6DKK2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MAQPHTTSVP YFARSPAPPP PSRSGAPPQP PATLRPSRRR TRPPRPADRR DAPADCAYLW
61RILTPRRGRA RRSDVGARHR ACGRRDVLLS RQGPANPEGA RRVVGGQERV WPAVRRGRGG
121SMFRLLSWSL GRGFLRAAGR RCRGCSARLL PGLAGGPGPE VQVPPSRVAP HGRGPGLLPL
181LAALAWFSRP AAAEEEEQQG ADGAAAEDGA DEAEAEIIQL LKRAKLSIMK DEPEEAELIL
241HDALRLAYQT DNKKAITYTY DLMANLAFIR GQLENAEQLF KATMSYLLGG GMKQEDNAII
301EISLKLASIY AAQNRQEFAV AGYEFCISTL EEKIEREKEL AEDIMSVEEK ANTHLLLGMC
361LDACARYLLF SKQPSQAQRM YEKALQISEE IQGERHPQTI VLMSDLATTL DAQGRFDEAY
421IYMQRASDLA RQINHPELHM VLSNLAAVLM HRERYTQAKE IYQEALKQAK LKKDEISVQH
481IREELAELSK KSRPLTNSVK L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

501 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.