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TTC17 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC17 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC17 antibodies →
$504.00each
Catalog Number: LS-G111874-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC17 (NM_018259), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TPR repeat protein 17 | TTC17
Origin Species
Human
NCBI GenBank
NM_018259
SwissProt
Q96AE7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAVGVRGR YELPPCSGPG WLLSLSALLS VAARGAFATT HWVVTEDGKI QQQVDSPMNL
61KHPHDLVILM RQEATVNYLK ELEKQLVAQK IHIEENEDRD TGLEQRHNKE DPDCIKAKVP
121LGDLDLYDGT YITLESKDIS PEDYIDTESP VPPDPEQPDC TKILELPYSI HAFQHLRGVQ
181ERVNLSAPLL PKEDPIFTYL SKRLGRSIDD IGHLIHEGLQ KNTSSWVLYN MASFYWRIKN
241EPYQVVECAM RALHFSSRHN KDIALVNLAN VLHRAHFSAD AAVVVHAALD DSDFFTSYYT
301LGNIYAMLGE YNHSVLCYDH ALQARPGFEQ AIKRKHAVLC QQKLEQKLEA QHRSLQRTLN
361ELKEYQKQHD HYLRQQEILE KHKLIQEEQI LRNIIHETQM AKEAQLGNHQ ICRLVNQQHS
421LHCQWDQPVR YHRGDIFENV DYVQFGEDSS TSSMMSVNFD VQSNQSDIND SVKSSPVAHS
481ILWIWGRDSD AYRDKQHILW PKRADCTESY PRVPVGGELP TYFLPPENKG LRIHELSSDD
541YSTEEEAQTP DCSITDFRKS HTLSYLVKEL EVRMDLKAKM PDDHARKILL SRINNYTIPE
601EEIGSFLFHA INKPNAPIWL ILNEAGLYWR AVGNSTFAIA CLQRALNLAP LQYQDVPLVN
661LANLLIHYGL HLDATKLLLQ ALAINSSEPL TFLSLGNAYL ALKNISGALE AFRQALKLTT
721KCPECENSLK LIRCMQFYPF LYNITSSVCS GTVVEESNGS DEMENSDETK MSEEILALVD
781EFQQAWPLEG FGGALEMKGR RLDLQGIRVL KKGPQDGVAR SSCYGDCRSE DDEATEWITF
841QVKRVKKPKG DHKKTPGKKV ETGQIENGHR YQANLEITGP KVASPGPQGK KRDYQRLGWP
901SPDECLKLRW VELTAIVSTW LAVSSKNIDI TEHIDFATPI QQPAMEPLCN GNLPTSMHTL
961DHLHGVSNRA SLHYTGESQL TEVLQNLGKD QYPQQSLEQI GTRIAKVLEK NQTSWVLSSM
1021AALYWRVKGQ GKKAIDCLRQ ALHYAPHQMK DVPLISLANI LHNAKLWNDA VIVATMAVEI
1081APHFAVNHFT LGNVYVAMEE FEKALVWYES TLKLQPEFVP AKNRIQTIQC HLMLKKGRRS
1141P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1141 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.