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TTC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC14 antibodies →
$504.00each
Catalog Number: LS-G107505-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC14 (NM_133462), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TTC14 | DRDL5813 | KIAA1980 | PRO19630 | TPR repeat protein 14
Origin Species
Human
SwissProt
Q96N46

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRDLLRQSL NCHGSSLLSL LRSEQQDNPH FRSLLGSAAE PARGPPPQHP LQGRKEKRVD
61NIEIQKFISK KADLLFALSW KSDAPATSEI NEDSEDHYAI MPPLEQFMEI PSMDRRELFF
121RDIERGDIVI GRISSIREFG FFMVLICLGS GIMRDIAHLE ITALCPLRDV PSHSNHGDPL
181SYYQTGDIIR AGIKDIDRYH EKLAVSLYSS SLPPHLSGIK LGVISSEELP LYYRRSVELN
241SNSLESYENV MQSSLGFVNP GVVEFLLEKL GIDESNPPSL MRGLQSKNFS EDDFASALRK
301KQSASWALKC VKIGVDYFKV GRHVDAMNEY NKALEIDKQN VEALVARGAL YATKGSLNKA
361IEDFELALEN CPTHRNARKY LCQTLVERGG QLEEEEKFLN AESYYKKALA LDETFKDAED
421ALQKLHKYMQ KSLELREKQA EKEEKQKTKK IETSAEKLRK LLKEEKRLKK KRRKSTSSSS
481VSSADESVSS SSSSSSSGHK RHKKHKRNRS ESSRSSRRHS SRASSNQIDQ NRKDECYPVP
541ANTSASFLNH KQEVEKLLGK QDRLQYEKTQ IKEKDRCPLS SSSLEIPDDF GGRSEDPRDF
601YNSYKTQAGS SKTEKPYKSE RHFSSRRNSS DSFCRNSEDK IYGYRRFEKD IEGRKEHYRR
661WEPGSVRHST SPASSEYSWK SVEKYKKYAH SGSRDFSRHE QRYRLNTNQG EYEREDNYGE
721DIKTEVPEED ALSSKEHSES SVKKNLPQNL LNIFNQIAEF EKEKGNKSKN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

770 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.