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TTC13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC13 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC13 antibodies →
$725.00each
Catalog Number: LS-G109957-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC13 (NM_024525), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TPR repeat protein 13 | TTC13
Origin Species
Human
NCBI GenBank
NM_024525
SwissProt
Q8NBP0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPAGCCCCC CFWGGAVAAA GAARRVLLLL LLGVLSAGLR PGALATEHYS PLSLLKQELQ
61HRQQQEAPAG GGGCSPQSGD WGDQYSAECG ESSFLNFHDS DCEPKGSSPC DSLLSLNTEK
121ILSQAKSIAE QKRFPFATDN DSTNEELAIA YVLIGSGLYD EAIRHFSTML QEEPDLVSAI
181YGRGIAYGKK GLHDIKNAEL ALFELSRVIT LEPDRPEVFE QRAEILSPLG RINEAVNDLT
241KAIQLQPSAR LYRHRGTLYF ISEDYATAHE DFQQSLELNK NQPIAMLYKG LTFFHRGLLK
301EAIESFKEAL KQKVDFIDAY KSLGQAYREL GNFEAATESF QKALLLNQNH VQTLQLRGMM
361LYHHGSLQEA LKNFKRCLQL EPYNEVCQYM KGLSHVAMGQ FYEGIKAQTK VMLNDPLPGQ
421KASPEYLKVK YLREYSRHLH AHLDTPLTEY NIDVDLPGSF KDHWAKNLPF LIEDYEEQPG
481LQPHIKDVLH QNFESYKPEV QELICVADRL GSLMQYETPG FLPNKRIHRA MGLAALEVMQ
541AVQRTWTNSK VRMNGKTRLM QWRDMFDIAV KWRRIADPDQ PVLWLDQMPA RSLSRGFNNH
601INLIRGQVIN MRYLEYFEKI LHFIKDRILV YHGANNPKGL LEVREALEKV HKVEDLLPIM
661KQFNTKTKDG FTVNTKVPSL KDQGKEYDGF TITITGDKVG NILFSVETQT TEERTQLYHA
721EIDALYKDLT AKGKVLILSS EFGEADAVCN LILSLVYYFY NLMPLSRGSS VIAYSVIVGA
781LMASGKEVAG KIPKGKLVDF EAMTAPGSEA FSKVAKSWMN LKSISPSYKT LPSVSETFPT
841LRSMIEVLNT DSSPRCLKKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

860 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.