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TTC12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TTC12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TTC12 antibodies →
$725.00each
Catalog Number: LS-G112167-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TTC12 (NM_017868), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TPARM | TTC12 | TPR repeat protein 12
Origin Species
Human
NCBI GenBank
NM_017868
SwissProt
Q9H892

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDADKEKDLQ KFLKNVDEIS NLIQEMNSDD PVVQQKAVLE TEKRLLLMEE DQEEDECRTT
61LNKTMISPPQ TAMKSAEEIN SEAFLASVEK DAKERAKRRR ENKVLADALK EKGNEAFAEG
121NYETAILRYS EGLEKLKDMK VLYTNRAQAY MKLEDYEKAL VDCEWALKCD EKCTKAYFHM
181GKANLALKNY SVSRECYKKI LEINPKLQTQ VKGYLNQVDL QEKADLQEKE AHELLDSGKN
241TAVTTKNLLE TLSKPDQIPL FYAGGIEILT EMINECTEQT LFRMHNGFSI ISDNEVIRRC
301FSTAGNDAVE EMVCVSVLKL WQAVCSRNEE NQRVLVIHHD RARLLAALLS SKVLAIRQQS
361FALLLHLAQT ESGRSLIINH LDLTRLLEAL VSFLDFSDKE ANTAMGLFTD LALEERFQVW
421FQANLPGVLP ALTGVLKTDP KVSSSSALCQ CIAIMGNLSA EPTTRRHMAA CEEFGDGCLS
481LLARCEEDVD LFREVIYTLL GLMMNLCLQA PFVSEVWAVE VSRRCLSLLN SQDGGILTRA
541AGVLSRTLSS SLKIVEEALR AGVVKKMMKF LKTGGETASR YAIKILAICT NSYHEAREEV
601IRLDKKLSVM MKLLSSEDEV LVGNAALCLG NCMEVPNVAS SLLKTDLLQV LLKLAGSDTQ
661KTAVQVNAGI ALGKLCTAEP RFAAQLRKLH GLEILNSTMK YISDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

705 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.