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TSTD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TSTD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107033-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSTD2 (NM_139246), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C9orf97 | RP11-244N9.5 | PP4189 | TSTD2
Origin Species
Human
NCBI GenBank
AF258575
SwissProt
Q5T7W7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLINPSSSL KAELDGSTKK KYSFAKKKAF ALFVKTKEVP TKRSFECKEK LWKCCQQLFT
61DQTSIHRHVA TQHADEIYHQ TASILKQLAV TLSTSKSLSS ADEKNPLKEC LPHSHDVSAW
121LPDISCFNPD ELISGQGSEE GEVLLYYCYH DLEDPQWICA WQTALCQHLH LTGKIRIAAE
181GINGTVGGSK LATRLYVEVM LSFPLFKDDL CKDDFKTSKG GAHCFPELRV GVFEEIVPMG
241ISPKKISYKK PGIHLSPGEF HKEVEKFLSQ ANQEQSDTIL LDCRNFYESK IGRFQGCLAP
301DIRKFSYFPS YVDKNLELFR EKRVLMYCTG GIRCERGSAY LKAKGVCKEV FQLKGGIHKY
361LEEFPDGFYK GKLFVFDERY ALSYNSDVVS ECSYCGARWD QYKLCSTPQC RQLVLTCPAC
421QGQGFTACCV TCQDKGSRKV SGPMQDSFKE ECECTARRPR IPRELLQHVR QPVSPEPGPD
481ADEDGPVLM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

489 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.