Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TSSK4 / TSSK5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G129641-100
Catalog Number: LS-G129641
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSSK4 (NM_001184739), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C14orf20 | Serine/threonine kinase 22E | STK22E | Testis-specific kinase 4 | TSSK4 | TSK-4 | TSSK-4 | TSSK5 | TSK4
- Origin Species
- Human
- NCBI GenBank
- AX394713
- SwissProt
- Q6SA08
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGKGDVLEAA PTTTAYHSLM DEYGYEVGKA IGHGSYGSVY EAFYTKQKVM VAVKIISKKK61ASDDYLNKFL PREIQVMKVL RHKYLINFYR AIESTSRVYI ILELAQGGDV LEWIQRYGAC121SEPLAGKWFS QLTLGIAYLH SKSIVHRLMP SLSAAGRDLK LENLLLDKWE NVKISDFGFA181KMVPSNQPVG CSPSYRQVNC FSHLSQTYCG SFAYACPEIL RGLPYNPFLS DTWSMGVILY241TLVVAHLPFD DTNLKKLLRE TQKEVTFPAN HTISQECKNL ILQMLRQATK RATILDIIKD301SWVLKFQPEQ PTHEIRLLEA MCQLHNTTKQ HQSLQITTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
338 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.