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TSR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TSR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111957-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSR1 (NM_018128), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TSR1 | KIAA1401
Origin Species
Human
NCBI GenBank
NM_018128
SwissProt
Q2NL82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAHRPGPLK QQNKAHKGGR HRGRGSAQRD GKGRLALKTL SKKVRKELSR VDQRHRASQL
61RKQKKEAVLA EKRQLGGKDG PPHQVLVVPL HSRISLPEAM QLLQDRDTGT VHLNELGNTQ
121NFMLLCPRLK HRWFFTSARP GDLHVVLDMA KVADTILFLL DPLEGWDSTG DYCLSCLFAQ
181GLPTYTLAVQ GISGLPLKKQ IDTRKKLSKA VEKRFPHDKL LLLDTQQEAG MLLRQLANQK
241QQHLAFRDRR AYLFAHAVDF VPSEENNLVG TLKISGYVRG QTLNVNRLLH IVGYGDFQMK
301QIDAPGDPFP LNPRGIKPQK DPDMAMEICA TDAVDDMEEG LKVLMKADPG RQESLQAEVI
361PDPMEGEQTW PTEEELSEAK DFLKESSKVV KKVPKGTSSY QAEWILDGGS QSGGEGDEYE
421YDDMEHEDFM EEESQDESSE EEEEYETMTI GESVHDDLYD KKVDEEAEAK MLEKYKQERL
481EEMFPDEVDT PRDVAARIRF QKYRGLKSFR TSPWDPKENL PQDYARIFQF QNFTNTRKSI
541FKEVEEKEVE GAEVGWYVTL HVSEVPVSVV ECFRQGTPLI AFSLLPHEQK MSVLNMVVRR
601DPGNTEPVKA KEELIFHCGF RRFRASPLFS QHTAADKHKL QRFLTADMAL VATVYAPITF
661PPASVLLFKQ KSNGMHSLIA TGHLMSVDPD RMVIKRVVLS GHPFKIFTKM AVVRYMFFNR
721EDVLWFKPVE LRTKWGRRGH IKEPLGTHGH MKCSFDGKLK SQDTVLMNLY KRVFPKWTYD
781PYVPEPVPWL KSEISSTVPQ GGME
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

804 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.